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Title: Association of heterotrophic bacteria with aggregated Arthrospira platensis exopolysaccharides: implications in the induction of axenic cultures.
Authors: Shiraishi, Hideaki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3490-9269 (unconfirmed)
Author's alias: 白石, 英秋
Keywords: cyanobacteria
Arthrospira platensis
exopolysaccharides
axenic cultures
Alcian blue
Issue Date: 21-Oct-2014
Publisher: Taylor & Francis Group
Journal title: Bioscience, biotechnology, and biochemistry
Volume: 79
Issue: 2
Start page: 331
End page: 341
Abstract: Inducing an axenic culture of the edible cyanobacterium Arthrospira (Spirulina) platensis using differential filtration alone is never successful; thus, it has been thought that, in non-axenic cultures, a portion of contaminating bacteria is strongly associated with Arthrospira cells. However, examination of the behavior of these bacteria during filtration revealed that they were not associated with Arthrospira cells but with aggregates of exopolysaccharides present in the medium away from the Arthrospira cells. Based on this finding, a rapid and reliable method for preparing axenic trichomes of A. platensis was established. After verifying the axenicity of the resulting trichomes on enriched agar plates, they were individually transferred to fresh sterile medium using a handmade tool, a microtrowel, to produce axenic cultures. With this technique, axenic cultures of various A. platensis strains were successfully produced. The technique described in this study is potentially applicable to a wider range of filamentous cyanobacteria.
Rights: The Version of Record of this manuscript has been published and is available in Bioscience, Biotechnology, and Biochemistry (2015) http://www.tandfonline.com/10.1080/09168451.2014.972333
許諾条件により、本文ファイルは2015-10-21に公開.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/197924
DOI(Published Version): 10.1080/09168451.2014.972333
PubMed ID: 25333502
Appears in Collections:Journal Articles

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