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タイトル: | Yeast nitrogen utilization in the phyllosphere during plant lifespan under regulation of autophagy. |
著者: | Shiraishi, Kosuke https://orcid.org/0000-0001-8780-9053 (unconfirmed) Oku, Masahide https://orcid.org/0000-0001-5991-6281 (unconfirmed) Kawaguchi, Kosuke Uchida, Daichi Yurimoto, Hiroya https://orcid.org/0000-0001-7506-6184 (unconfirmed) Sakai, Yasuyoshi |
著者名の別形: | 阪井, 康能 |
キーワード: | Cellular microbiology Autophagy |
発行日: | 21-Apr-2015 |
出版者: | Nature Publishing Group |
誌名: | Scientific reports |
巻: | 5 |
論文番号: | 9719 |
抄録: | Recently, microbe-plant interactions at the above-ground parts have attracted great attention. Here we describe nitrogen metabolism and regulation of autophagy in the methylotrophic yeast Candida boidinii, proliferating and surviving on the leaves of Arabidopsis thaliana. After quantitative analyses of yeast growth on the leaves of A. thaliana with the wild-type and several mutant yeast strains, we showed that on young leaves, nitrate reductase (Ynr1) was necessary for yeast proliferation, and the yeast utilized nitrate as nitrogen source. On the other hand, a newly developed methylamine sensor revealed appearance of methylamine on older leaves, and methylamine metabolism was induced in C. boidinii, and Ynr1 was subjected to degradation. Biochemical and microscopic analysis of Ynr1 in vitro during a shift of nitrogen source from nitrate to methylamine revealed that Ynr1 was transported to the vacuole being the cargo for biosynthetic cytoplasm-to-vacuole targeting (Cvt) pathway, and degraded. Our results reveal changes in the nitrogen source composition for phyllospheric yeasts during plant aging, and subsequent adaptation of the yeasts to this environmental change mediated by regulation of autophagy. |
著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
URI: | http://hdl.handle.net/2433/198437 |
DOI(出版社版): | 10.1038/srep09719 |
PubMed ID: | 25900611 |
出現コレクション: | 学術雑誌掲載論文等 |
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