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Title: Yeast nitrogen utilization in the phyllosphere during plant lifespan under regulation of autophagy.
Authors: Shiraishi, Kosuke  kyouindb  KAKEN_id  orcid (unconfirmed)
Oku, Masahide  kyouindb  KAKEN_id  orcid (unconfirmed)
Kawaguchi, Kosuke
Uchida, Daichi
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid (unconfirmed)
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Author's alias: 阪井, 康能
Keywords: Cellular microbiology
Issue Date: 21-Apr-2015
Publisher: Nature Publishing Group
Journal title: Scientific reports
Volume: 5
Thesis number: 9719
Abstract: Recently, microbe-plant interactions at the above-ground parts have attracted great attention. Here we describe nitrogen metabolism and regulation of autophagy in the methylotrophic yeast Candida boidinii, proliferating and surviving on the leaves of Arabidopsis thaliana. After quantitative analyses of yeast growth on the leaves of A. thaliana with the wild-type and several mutant yeast strains, we showed that on young leaves, nitrate reductase (Ynr1) was necessary for yeast proliferation, and the yeast utilized nitrate as nitrogen source. On the other hand, a newly developed methylamine sensor revealed appearance of methylamine on older leaves, and methylamine metabolism was induced in C. boidinii, and Ynr1 was subjected to degradation. Biochemical and microscopic analysis of Ynr1 in vitro during a shift of nitrogen source from nitrate to methylamine revealed that Ynr1 was transported to the vacuole being the cargo for biosynthetic cytoplasm-to-vacuole targeting (Cvt) pathway, and degraded. Our results reveal changes in the nitrogen source composition for phyllospheric yeasts during plant aging, and subsequent adaptation of the yeasts to this environmental change mediated by regulation of autophagy.
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit
DOI(Published Version): 10.1038/srep09719
PubMed ID: 25900611
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