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Title: ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.
Authors: Oomoto, Ikumi
Suzuki-Hirano, Asuka
Umeshima, Hiroki
Han, Yong-Woon
Yanagisawa, Hiroyuki
Carlton, Peter  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5320-6024 (unconfirmed)
Harada, Yoshie
Kengaku, Mineko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-1666-3648 (unconfirmed)
Okamoto, Akimitsu
Shimogori, Tomomi
Wang, Dan Ohtan
Author's alias: 原田, 慶恵
見学, 美根子
王, 丹
Issue Date: 22-Jun-2015
Publisher: Oxford University Press
Journal title: Nucleic acids research
Volume: 43
Issue: 19
Thesis number: e126
Abstract: Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. Here, we describe a high-resolution fluorescence RNA imaging method, ECHO-liveFISH, to label endogenous nuclear RNA in living mice and chicks. Upon in vivo electroporation, exciton-controlled sequence-specific oligonucleotide probes revealed focally concentrated endogenous 28S rRNA and U3 snoRNA at nucleoli and poly(A) RNA at nuclear speckles. Time-lapse imaging reveals steady-state stability of these RNA foci and dynamic dissipation of 28S rRNA concentrations upon polymerase I inhibition in native brain tissue. Confirming the validity of this technique in a physiological context, the in vivo RNA labeling did not interfere with the function of target RNA nor cause noticeable cytotoxicity or perturbation of cellular behavior.
Description: 生きたマウス脳内の細胞内RNA活動の可視化に成功 -早くて正確な製薬時のスクリーニングなど応用に期待-. 京都大学プレスリリース. 2015-06-29.
Rights: © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/198594
DOI(Published Version): 10.1093/nar/gkv614
PubMed ID: 26101260
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2015-06-29-0
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