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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| anie.201410339.pdf | 470.62 kB | Adobe PDF | 見る/開く |
| タイトル: | Live-cell imaging of endogenous mRNAs with a small molecule. |
| 著者: | Sato, Shin-ichi Watanabe, Mizuki Katsuda, Yousuke Murata, Asako Wang, Dan Ohtan Uesugi, Motonari    https://orcid.org/0000-0002-8515-445X (unconfirmed) |
| 著者名の別形: | 佐藤, 慎一 |
| キーワード: | fluorescence live-cell imaging RNA RNA dynamics small molecules |
| 発行日: | 23-Dec-2014 |
| 出版者: | wiley |
| 誌名: | Angewandte Chemie |
| 巻: | 54 |
| 号: | 6 |
| 開始ページ: | 1855 |
| 終了ページ: | 1858 |
| 抄録: | Determination of subcellular localization and dynamics of mRNA is increasingly important to understanding gene expression. A new convenient and versatile method is reported that permits spatiotemporal imaging of specific non-engineered RNAs in living cells. The method uses transfection of a plasmid encoding a gene-specific RNA aptamer, combined with a cell-permeable synthetic small molecule, the fluorescence of which is restored only when the RNA aptamer hybridizes with its cognitive mRNA. The method was validated by live-cell imaging of the endogenous mRNA of β-actin. Application of the technology to mRNAs of a total of 84 human cytoskeletal genes allowed us to observe cellular dynamics of several endogenous mRNAs including arfaptin-2, cortactin, and cytoplasmic FMR1-interacting protein 2. The RNA-imaging technology and its further optimization might permit live-cell imaging of any RNA molecules. |
| 著作権等: | This is the peer reviewed version of the following article: Sato, S.-i., Watanabe, M., Katsuda, Y., Murata, A., Wang, D. O. and Uesugi, M. (2015), Live-Cell Imaging of Endogenous mRNAs with a Small Molecule. Angew. Chem. Int. Ed., 54: 1855–1858, which has been published in final form at http://dx.doi.org/10.1002/anie.201410339. 許諾条件により本文ファイルは2015-12-23に公開. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
| URI: | http://hdl.handle.net/2433/198615 |
| DOI(出版社版): | 10.1002/anie.201410339 |
| PubMed ID: | 25537455 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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