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タイトル: | Sequence-specific DNA recognition by cyclic pyrrole-imidazole cysteine-derived polyamide dimers. |
著者: | Yamamoto, Makoto Bando, Toshikazu Morinaga, Hironobu Kawamoto, Yusuke https://orcid.org/0000-0001-9655-8241 (unconfirmed) Hashiya, Kaori Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
著者名の別形: | 杉山, 弘 |
キーワード: | dimerization DNA recognition molecular recognition nucleic acids oligonucleotides |
発行日: | 11-Dec-2013 |
出版者: | wiley |
誌名: | Chemistry - A European Journal |
巻: | 20 |
号: | 3 |
開始ページ: | 752 |
終了ページ: | 759 |
抄録: | Pyrrole-imidazole (PI) polyamides bind to the minor groove of the DNA duplex in a sequence-specific manner and thus have the potential to regulate gene expression. To date, various types of PI polyamides have been designed as sequence-specific DNA binding ligands. One of these, cysteine cyclic PI polyamides containing two β-alanine molecules, were designed to recognize a 7 bp DNA sequence with high binding affinity. In this study, an efficient cyclization reaction between a cysteine and a chloroacetyl residue was used for dimerization in the synthesis of a unit that recognizes symmetrical DNA sequences. To evaluate specific DNA binding properties, dimeric PI polyamide binding was measured by using a surface plasmon resonance (SPR) method. Extending this molecular design, we synthesized a large dimeric PI polyamide that can recognize a 14 bp region in duplex DNA. |
著作権等: | This is the peer reviewed version of the following article: Yamamoto, M., Bando, T., Morinaga, H., Kawamoto, Y., Hashiya, K. and Sugiyama, H. (2014), Sequence-Specific DNA Recognition by Cyclic Pyrrole–Imidazole Cysteine-Derived Polyamide Dimers. Chem. Eur. J., 20: 752–759, which has been published in final form athttp://dx.doi.org/10.1002/chem.201302482. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/198837 |
DOI(出版社版): | 10.1002/chem.201302482 |
PubMed ID: | 24339258 |
出現コレクション: | 学術雑誌掲載論文等 |
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