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タイトル: | Sodium-driven energy conversion for flagellar rotation of the earliest divergent hyperthermophilic bacterium |
著者: | Takekawa, Norihiro Nishiyama, Masayoshi Kaneseki, Tsuyoshi Kanai, Tamotsu Atomi, Haruyuki https://orcid.org/0000-0001-9687-6426 (unconfirmed) Kojima, Seiji Homma, Michio |
著者名の別形: | 竹川, 宜宏 西山, 雅祥 金井, 保 本間, 道夫 |
キーワード: | Bacteria Bioenergetics |
発行日: | 5-Aug-2015 |
出版者: | Nature Publishing Group |
誌名: | Scientific Reports |
巻: | 5 |
論文番号: | 12711 |
抄録: | Aquifex aeolicus is a hyperthermophilic, hydrogen-oxidizing and carbon-fixing bacterium that can grow at temperatures up to 95 °C. A. aeolicus has an almost complete set of flagellar genes that are conserved in bacteria. Here we observed that A. aeolicus has polar flagellum and can swim with a speed of 90 μm s[−1] at 85 °C. We expressed the A. aeolicus mot genes (motA and motB), which encode the torque generating stator proteins of the flagellar motor, in a corresponding motnonmotile mutant of Escherichia coli. Its motility was slightly recovered by expression of A. aeolicus MotA and chimeric MotB whose periplasmic region was replaced with that of E. coli. A point mutation in the A. aeolicus MotA cytoplasmic region remarkably enhanced the motility. Using this system in E. coli, we demonstrate that the A. aeolicus motor is driven by Na[+]. As motor proteins from hyperthermophilic bacteria represent the earliest motor proteins in evolution, this study strongly suggests that ancient bacteria used Na[+] for energy coupling of the flagellar motor. The Na[+]-driven flagellar genes might have been laterally transferred from early-branched bacteria into late-branched bacteria and the interaction surfaces of the stator and rotor seem not to change in evolution. |
記述: | 細菌の祖先はナトリウムを使ってエネルギー変換 -原始のモーターを現代で再現する-. 京都大学プレスリリース. 2015-08-24. |
著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
URI: | http://hdl.handle.net/2433/198908 |
DOI(出版社版): | 10.1038/srep12711 |
PubMed ID: | 26244427 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2015-08-24 |
出現コレクション: | 学術雑誌掲載論文等 |
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