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Title: Structure and mechanism of the mammalian fructose transporter GLUT5.
Authors: Nomura, Norimichi  kyouindb  KAKEN_id
Verdon, Grégory
Kang, Hae Joo
Shimamura, Tatsuro  kyouindb  KAKEN_id
Nomura, Yayoi
Sonoda, Yo
Hussien, Saba Abdul
Qureshi, Aziz Abdul
Coincon, Mathieu
Sato, Yumi
Abe, Hitomi
Nakada-Nakura, Yoshiko
Hino, Tomoya
Arakawa, Takatoshi
Kusano-Arai, Osamu
Iwanari, Hiroko
Murata, Takeshi
Kobayashi, Takuya
Hamakubo, Takao
Kasahara, Michihiro
Iwata, So  kyouindb  KAKEN_id
Drew, David
Author's alias: 野村, 紀通
岩田, 想
Issue Date: 30-Sep-2015
Publisher: Nature Publishing Group
Journal title: Nature
Volume: 526
Issue: 7573
Start page: 397
End page: 401
Abstract: The altered activity of the fructose transporter GLUT5, an isoform of the facilitated-diffusion glucose transporter family, has been linked to disorders such as type 2 diabetes and obesity. GLUT5 is also overexpressed in certain tumour cells, and inhibitors are potential drugs for these conditions. Here we describe the crystal structures of GLUT5 from Rattus norvegicus and Bos taurus in open outward- and open inward-facing conformations, respectively. GLUT5 has a major facilitator superfamily fold like other homologous monosaccharide transporters. On the basis of a comparison of the inward-facing structures of GLUT5 and human GLUT1, a ubiquitous glucose transporter, we show that a single point mutation is enough to switch the substrate-binding preference of GLUT5 from fructose to glucose. A comparison of the substrate-free structures of GLUT5 with occluded substrate-bound structures of Escherichia coli XylE suggests that, in addition to global rocker-switch-like re-orientation of the bundles, local asymmetric rearrangements of carboxy-terminal transmembrane bundle helices TM7 and TM10 underlie a 'gated-pore' transport mechanism in such monosaccharide transporters.
Description: 糖分を細胞内に輸送する膜たんぱく質の立体構造と動きを解明 -肥満やがんの抑制策に役立つ新たな知見-. 京都大学プレスリリース. 2015-10-01.
Rights: © 2015 Macmillan Publishers Limited.
The full-text file will be made open to the public on 30 march 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/199936
DOI(Published Version): 10.1038/nature14909
PubMed ID: 26416735
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2015/151001_1.html
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