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Title: SecA defects are accompanied by dysregulation of MukB, DNA gyrase, chromosome partitioning and DNA superhelicity in Escherichia coli.
Authors: Adachi, Shun
Murakawa, Yasuhiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5415-1352 (unconfirmed)
Hiraga, Sota
Author's alias: 足立, 隼
村川, 泰裕
平賀, 壯太
Issue Date: 1-Aug-2014
Publisher: Microbiology Society
Journal title: Microbiology
Volume: 160
Start page: 1648
End page: 1658
Abstract: Spatial regulation of nucleoids and chromosome-partitioning proteins is important for proper chromosome partitioning in Escherichia coli. However, the underlying molecular mechanisms are unknown. In the present work, we showed that mutation or chemical perturbation of secretory A (SecA), an ATPase component of the membrane protein translocation machinery, SecY, a component of the membrane protein translocation channel and acyl carrier protein P (AcpP), which binds to SecA and MukB, a functional homologue of structural maintenance of chromosomes protein (SMC), resulted in a defect in chromosome partitioning. We further showed that SecA is essential for proper positioning of the oriC DNA region, decatenation and maintenance of superhelicity of DNA. Genetic interaction studies revealed that the topological abnormality observed in the secA mutant was due to combined inhibitory effects of defects in MukB, DNA gyrase and Topo IV, suggesting a role for the membrane protein translocation machinery in chromosome partitioning and/or structural maintenance of chromosomes.
Rights: © 2014 The Authors. Published by the Microbiology Society.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/200187
DOI(Published Version): 10.1099/mic.0.077685-0
PubMed ID: 24858081
Appears in Collections:Journal Articles

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