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タイトル: | Tobacco streak virus (strain dahlia) suppresses post-transcriptional gene silencing of flavone synthase II in black dahlia cultivars and causes a drastic flower color change. |
著者: | Deguchi, Ayumi Tatsuzawa, Fumi Hosokawa, Munetaka Doi, Motoaki Ohno, Sho https://orcid.org/0000-0002-0810-0327 (unconfirmed) |
著者名の別形: | 大野, 翔 |
キーワード: | Cyanidin Dahlia variabilis FNS II PTGS Silencing suppressor |
発行日: | 18-Jul-2015 |
出版者: | Springer Berlin Heidelberg |
誌名: | Planta |
巻: | 242 |
号: | 3 |
開始ページ: | 663 |
終了ページ: | 675 |
抄録: | Tobacco streak virus suppressed post-transcriptional gene silencing and caused a flower color change in black dahlias, which supported the role of cyanidin-based anthocyanins for black flower appearance. Black flower color of dahlia (Dahlia variabilis) has been attributed, in part, to the high accumulation of cyanidin-based anthocyanins that occurs when flavone synthesis is reduced because of post-transcriptional gene silencing (PTGS) of flavone synthase II (DvFNS). There are also purple-flowering plants that have emerged from a black cultivar 'Kokucho'. We report that the purple color is not caused by a mutation, as previously thought, but by infection with tobacco streak virus (TSVdahlia), which suppresses the PTGS of DvFNS. When TSVdahlia was eliminated from the purple-flowering 'Kokucho' by leaf primordia-free shoot apical meristem culture, the resulting flowers were black. TSVdahlia-infected purple flowers had lower numbers of siRNAs to DvFNS than black flowers, suggesting that TSVdahlia has a silencing suppressor. The graft inoculation of other black cultivars with TSVdahlia altered their flower color drastically except for 'Fidalgo Blacky', a very deep black cultivar with the highest amount of cyanidin-based anthocyanins. The flowers of all six TSVdahlia-infected cultivars accumulated increased amounts of flavones and reduced amounts of cyanidin-based anthocyanins. 'Fidalgo Blacky' remained black despite the change in pigment accumulation, and the amounts of cyanidin-based anthocyanins in its TSVdahlia-infected plants were still higher than those of other cultivars. We propose that black flower color in dahlia is controlled by two different mechanisms that increase the amount of cyanidin-based anthocyanins: DvFNS PTGS-dependent and -independent mechanisms. If both mechanisms occur simultaneously, the flower color will be blacker than if only a single mechanism is active. |
著作権等: | The final publication is available at Springer via http://dx.doi.org/10.1007/s00425-015-2365-6. The full-text file will be made open to the public on 18 July 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/201879 |
DOI(出版社版): | 10.1007/s00425-015-2365-6 |
PubMed ID: | 26186968 |
出現コレクション: | 学術雑誌掲載論文等 |
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