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dc.contributor.author | Miyashita, Masahiro | en |
dc.contributor.author | Hanai, Yosuke | en |
dc.contributor.author | Awane, Hiroyuki | en |
dc.contributor.author | Yoshikawa, Toru | en |
dc.contributor.author | Miyagawa, Hisashi | en |
dc.contributor.alternative | 宮下, 正弘 | ja |
dc.date.accessioned | 2015-12-02T05:49:53Z | - |
dc.date.available | 2015-12-02T05:49:53Z | - |
dc.date.issued | 2011-05-15 | - |
dc.identifier.issn | 0951-4198 | - |
dc.identifier.uri | http://hdl.handle.net/2433/202058 | - |
dc.description | Article first published online: 12 APR 2011 | en |
dc.description.abstract | An improved method of de novo peptide sequencing based on mass spectrometry using novel N-terminal derivatization reagents with high proton affinity has been developed. The introduction of a positively charged group into the N-terminal amino group of a peptide is known to enhance the relative intensity of b-ions in product ion spectra, allowing the easy interpretation of the spectra. However, the physicochemical properties of charge derivatization reagents required for efficient fragmentation remain unclear. In this study, we prepared several derivatization reagents with high proton affinity, which are thought to be appropriate for peptide fragmentation under low-energy collision-induced dissociation (CID) conditions, and examined their usefulness in de novo peptide sequencing. Comparison of the effects on fragmentation among three derivatization reagents having a guanidino or an amidino moiety, which differ in proton affinity, clearly indicated that there was an optimal proton affinity for efficient fragmentation of peptides. Among reagents tested in this study, derivatization with 4-amidinobenzoic acid brought about the most effective fragmentation. This derivatization approach will offer a novel de novo peptide sequencing method under low-energy CID conditions. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | wiley | en |
dc.rights | This is the peer reviewed version of the following article: Miyashita, M., Hanai, Y., Awane, H., Yoshikawa, T. and Miyagawa, H. (2011), Improving peptide fragmentation by N-terminal derivatization with high proton affinity. Rapid Commun. Mass Spectrom., 25: 1130–1140, which has been published in final form at http://dx.doi.org/10.1002/rcm.4962. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject.mesh | Amino Acid Sequence | en |
dc.subject.mesh | Amino Acids, Basic/chemistry | en |
dc.subject.mesh | Benzoates/chemistry | en |
dc.subject.mesh | Glycolates/chemistry | en |
dc.subject.mesh | Guanidines/chemistry | en |
dc.subject.mesh | Hydrogen-Ion Concentration | en |
dc.subject.mesh | Mass Spectrometry | en |
dc.subject.mesh | Niacin/chemistry | en |
dc.subject.mesh | Peptide Fragments/chemistry | en |
dc.subject.mesh | Sequence Analysis, Protein/methods | en |
dc.title | Improving peptide fragmentation by N-terminal derivatization with high proton affinity. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA10704286 | - |
dc.identifier.jtitle | Rapid communications in mass spectrometry | en |
dc.identifier.volume | 25 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 1130 | - |
dc.identifier.epage | 1140 | - |
dc.relation.doi | 10.1002/rcm.4962 | - |
dc.textversion | author | - |
dc.startdate.bitstreamsavailable | 2012-04-12 | - |
dc.identifier.pmid | 21488112 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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