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dc.contributor.authorKanda, Junyaen
dc.contributor.authorMori, Kiyoshien
dc.contributor.authorKawabata, Hiroshien
dc.contributor.authorKuwabara, Takashigeen
dc.contributor.authorMori, Keita Pen
dc.contributor.authorImamaki, Hirotakaen
dc.contributor.authorKasahara, Masatoen
dc.contributor.authorYokoi, Hidekien
dc.contributor.authorMizumoto, Chisakien
dc.contributor.authorThoennissen, Nils Hen
dc.contributor.authorKoeffler, H Phillipen
dc.contributor.authorBarasch, Jonathanen
dc.contributor.authorTakaori-Kondo, Akifumien
dc.contributor.authorMukoyama, Masashien
dc.contributor.authorNakao, Kazuwaen
dc.contributor.alternative森, 潔ja
dc.date.accessioned2015-12-14T05:00:42Z-
dc.date.available2015-12-14T05:00:42Z-
dc.date.issued2015-02-
dc.identifier.issn1342-1751-
dc.identifier.urihttp://hdl.handle.net/2433/202580-
dc.descriptionFirst online: 06 March 2014en
dc.description.abstract[Background]Lipocalin 2 (LCN2 or neutrophil gelatinase-associated lipocalin) is a secretory protein discovered from neutrophils, which accumulates in the blood and urine during acute kidney injury (AKI) and in the blood by bacterial infection. Little is known about the tissue source and molecular forms of this protein under normal and pathophysiologic conditions. [Methods]By sandwich ELISA, serum and urinary LCN2 levels were measured in 36 patients with hematologic malignancies who transiently became neutropenic by stem cell transplantation (SCT). To evaluate contribution of neutrophil-derived LCN2 in the physiologic blood LCN2 concentrations, we examined CCAAT/enhancer-binding protein ε (C/EBPε) knockout mice, which lack mature neutrophils. [Results]In patients without AKI and bacterial infection, at 1 week after SCT, the median blood neutrophil counts became zero and serum LCN2 levels were decreased by 76 ± 6 % (p < 0.01), but urinary LCN2 levels were not altered. During neutropenic conditions, bacterial infection caused only a modest rise of serum LCN2 but AKI produced a marked rise of serum and urinary LCN2 levels. Serum LCN2 concentrations in C/EBPε knockout mice were reduced by 66 ± 11 % compared to wild-type mice (p < 0.05). Blood LCN2 existed predominantly in high molecular weight forms (>100 kDa), while urinary LCN2 was mainly in low molecular weight forms. [Conclusion]Our findings suggest that neutrophils are the major source of circulating LCN2 in normal and infected conditions, whereas blood and urinary LCN2 mainly derive from the kidney during AKI, and that the molecular forms and regulation of blood and urinary LCN2 are clearly distinct.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherSpringer Japanen
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/s10157-014-0952-7.en
dc.rightsThe full-text file will be made open to the public on 06 March 2015 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.en
dc.rightsこの論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.rightsThis is not the published version. Please cite only the published version.en
dc.subjectAcute kidney injuryen
dc.subjectNeutrophilen
dc.subjectSepsis, bone marrow transplantationen
dc.subjectBiomarkeren
dc.subject.meshAcute Kidney Injury/blooden
dc.subject.meshAcute-Phase Proteins/urineen
dc.subject.meshAnimalsen
dc.subject.meshBacterial Infections/blooden
dc.subject.meshBacterial Infections/urineen
dc.subject.meshBiological Markers/blooden
dc.subject.meshBiological Markers/urineen
dc.subject.meshCCAAT-Enhancer-Binding Proteins/geneticsen
dc.subject.meshCCAAT-Enhancer-Binding Proteins/physiologyen
dc.subject.meshHematologic Neoplasms/complicationsen
dc.subject.meshHematologic Neoplasms/therapyen
dc.subject.meshHematopoietic Stem Cell Transplantationen
dc.subject.meshHumansen
dc.subject.meshKidney/metabolismen
dc.subject.meshLipocalins/blooden
dc.subject.meshLipocalins/urineen
dc.subject.meshMiceen
dc.subject.meshMice, Knockouten
dc.subject.meshMolecular Weighten
dc.subject.meshNeutrophils/metabolismen
dc.subject.meshOncogene Proteins/blooden
dc.subject.meshOncogene Proteins/urineen
dc.titleAn AKI biomarker lipocalin 2 in the blood derives from the kidney in renal injury but from neutrophils in normal and infected conditions.en
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.ncidAA11126935-
dc.identifier.jtitleClinical and experimental nephrologyen
dc.identifier.volume19-
dc.identifier.issue1-
dc.identifier.spage99-
dc.identifier.epage106-
dc.relation.doi10.1007/s10157-014-0952-7-
dc.textversionauthor-
dc.startdate.bitstreamsavailable2015-03-06-
dc.identifier.pmid24599361-
dcterms.accessRightsopen access-
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