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タイトル: | Optimization of surface-immobilized extracellular matrices for the proliferation of neural progenitor cells derived from induced pluripotent stem cells. |
著者: | Komura, Takashi Kato, Koichi Konagaya, Shuhei Nakaji-Hirabayashi, Tadashi Iwata, Hiroo |
著者名の別形: | 小長谷, 周平 岩田, 博夫 |
キーワード: | extracellular matrix iPS cell neural progenitor cell laminin regenerative medicine |
発行日: | Nov-2015 |
出版者: | wiley |
誌名: | Biotechnology and bioengineering |
巻: | 112 |
号: | 11 |
開始ページ: | 2388 |
終了ページ: | 2396 |
抄録: | Neural progenitor cells derived from induced pluripotent stem cells have been considered as a potential source for cell-transplantation therapy of central nervous disorders. However, efficient methods to expand neural progenitor cells are further required for their clinical applications. In this study, a protein array was fabricated with nine extracellular matrices and used to screen substrates suitable for the expansion of neural progenitor cells derived from mouse induced pluripotent stem cells. The results showed that neural progenitor cells efficiently proliferated on substrates with immobilized laminin-1, laminin-5, or Matrigel. Based on this result, further attempts were made to develop clinically compliant substrates with immobilized polypeptides that mimic laminin-1, one of the most effective extracellular matrices as identified in the array-based screening. We used here recombinant DNA technology to prepare polypeptide containing the globular domain 3 of laminin-1 and immobilized it onto glass-based substrates. Our results showed that neural progenitor cells selectively proliferated on substrate with the immobilized polypeptide while maintaining their differentiated state. |
記述: | Article first published online: 20 MAY 2015 |
著作権等: | This is the peer reviewed version of the following article: Komura, T., Kato, K., Konagaya, S., Nakaji-Hirabayashi, T. and Iwata, H. (2015), Optimization of surface-immobilized extracellular matrices for the proliferation of neural progenitor cells derived from induced pluripotent stem cells. Biotechnol. Bioeng., 112: 2388–2396, which has been published in final form at http://dx.doi.org/10.1002/bit.25636. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. The full-text file will be made open to the public on 20 MAY 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/202837 |
DOI(出版社版): | 10.1002/bit.25636 |
PubMed ID: | 25943789 |
出現コレクション: | 学術雑誌掲載論文等 |
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