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Title: Gut Microbial Fatty Acid Metabolites Reduce Triacylglycerol Levels in Hepatocytes.
Authors: Nanthirudjanar, Tharnath
Furumoto, Hidehiro
Zheng, Jiawen
Kim, Young-Il
Goto, Tsuyoshi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-1283-147X (unconfirmed)
Takahashi, Nobuyuki
Kawada, Teruo
Park, Si-Bum
Hirata, Akiko
Kitamura, Nahoko
Kishino, Shigenobu  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-4587-2422 (unconfirmed)
Ogawa, Jun  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-2741-621X (unconfirmed)
Hirata, Takashi
Sugawara, Tatsuya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-1203-5521 (unconfirmed)
Author's alias: 菅原, 達也
Keywords: Acetyl-CoA carboxylase
Hydroxy fatty acid
Oxo fatty acid
Lipogenesis
Liver
Sterol regulatory element binding
protein-1c
Issue Date: Nov-2015
Publisher: Springer Berlin Heidelberg
Journal title: Lipids
Volume: 50
Issue: 11
Start page: 1093
End page: 1102
Abstract: Hydroxy and oxo fatty acids were recently found to be produced as intermediates during gut microbial fatty acid metabolism. Lactobacillus plantarum produces these fatty acids from unsaturated fatty acids such as linoleic acid. In this study, we investigated the effects of these gut microbial fatty acid metabolites on the lipogenesis in liver cells. We screened their effect on sterol regulatory element binding protein-1c (SREBP-1c) expression in HepG2 cells treated with a synthetic liver X receptor α (LXRα) agonist (T0901317). The results showed that 10-hydroxy-12(Z)-octadecenoic acid (18:1) (HYA), 10-hydroxy-6(Z), 12(Z)-octadecadienoic acid (18:2) (γHYA), 10-oxo-12(Z)-18:1 (KetoA), and 10-oxo-6(Z), 12(Z)-18:2 (γKetoA) significantly decreased SREBP-1c mRNA expression induced by T0901317. These fatty acids also downregulated the mRNA expression of lipogenic genes by suppressing LXRα activity and inhibiting SREBP-1 maturation. Oral administration of KetoA, which effectively reduced triacylglycerol accumulation and acetyl-CoA carboxylase 2 (ACC2) expression in HepG2 cells, for 2 weeks significantly decreased Srebp-1c, Scd-1, and Acc2 expression in the liver of mice fed a high-sucrose diet. Our findings suggest that the hypolipidemic effect of the fatty acid metabolites produced by L. plantarum can be exploited in the treatment of cardiovascular diseases or dyslipidemia.
Description: First online: 23 September 2015
Rights: The final publication is available at Springer via http://dx.doi.org/10.1007/s11745-015-4067-z.
The full-text file will be made open to the public on 23 September 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/203067
DOI(Published Version): 10.1007/s11745-015-4067-z
PubMed ID: 26399511
Appears in Collections:Journal Articles

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