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タイトル: | Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is Involved in Plasma Membrane Dynamics. |
著者: | Naito, Tomoki Takatsu, Hiroyuki Miyano, Rie Takada, Naoto Nakayama, Kazuhisa ![]() ![]() Shin, Hye-Won ![]() ![]() ![]() |
著者名の別形: | 申, 惠媛 |
キーワード: | ATPase lipid bilayer membrane protein phospholipid plasma membrane cell spreading flippase |
発行日: | 12-Jun-2015 |
出版者: | American Society for Biochemistry and Molecular Biology |
誌名: | The Journal of biological chemistry |
巻: | 290 |
号: | 24 |
開始ページ: | 15004 |
終了ページ: | 15017 |
抄録: | We showed previously that ATP11A and ATP11C have flippase activity toward aminophospholipids (phosphatidylserine (PS) and phosphatidylethanolamine (PE)) and ATP8B1 and that ATP8B2 have flippase activity toward phosphatidylcholine (PC) (Takatsu, H., Tanaka, G., Segawa, K., Suzuki, J., Nagata, S., Nakayama, K., and Shin, H. W. (2014) J. Biol. Chem. 289, 33543-33556). Here, we show that the localization of class 5 P4-ATPases to the plasma membrane (ATP10A and ATP10D) and late endosomes (ATP10B) requires an interaction with CDC50A. Moreover, exogenous expression of ATP10A, but not its ATPase-deficient mutant ATP10A(E203Q), dramatically increased PC flipping but not flipping of PS or PE. Depletion of CDC50A caused ATP10A to be retained at the endoplasmic reticulum instead of being delivered to the plasma membrane and abrogated the increased PC flipping activity observed by expression of ATP10A. These results demonstrate that ATP10A is delivered to the plasma membrane via its interaction with CDC50A and, specifically, flips PC at the plasma membrane. Importantly, expression of ATP10A, but not ATP10A(E203Q), dramatically altered the cell shape and decreased cell size. In addition, expression of ATP10A, but not ATP10A(E203Q), delayed cell adhesion and cell spreading onto the extracellular matrix. These results suggest that enhanced PC flipping activity due to exogenous ATP10A expression alters the lipid composition at the plasma membrane, which may in turn cause a delay in cell spreading and a change in cell morphology. |
著作権等: | This research was originally published in [The Journal of Biological Chemistry, 290, 15004-15017. doi: 10.1074/jbc.M115.655191] © the American Society for Biochemistry and Molecular Biology この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/203100 |
DOI(出版社版): | 10.1074/jbc.M115.655191 |
PubMed ID: | 25947375 |
出現コレクション: | 学術雑誌掲載論文等 |
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