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タイトル: | Crystal structure of a symbiosis-related lectin from octocoral. |
著者: | Kita, Akiko https://orcid.org/0000-0002-5174-6238 (unconfirmed) Jimbo, Mitsuru Sakai, Ryuichi Morimoto, Yukio https://orcid.org/0000-0003-1919-6309 (unconfirmed) Miki, Kunio |
著者名の別形: | 喜田, 昭子 |
キーワード: | coral-algae symbiosis coral lectin crystal structure signal transduction SLL-2 |
発行日: | Sep-2015 |
出版者: | Oxford University Press |
誌名: | Glycobiology |
巻: | 25 |
号: | 9 |
開始ページ: | 1016 |
終了ページ: | 1023 |
抄録: | D-Galactose-binding lectin from the octocoral, Sinularia lochmodes (SLL-2), distributes densely on the cell surface of microalgae, Symbiodinium sp., an endosymbiotic dinoflagellate of the coral, and is also shown to be a chemical cue that transforms dinoflagellate into a non-motile (coccoid) symbiotic state. SLL-2 binds with high affinity to the Forssman antigen (N-acetylgalactosamine(GalNAc)α1-3GalNAcβ1-3Galα1-4Galβ1-4Glc-ceramide), and the presence of Forssman antigen-like sugar on the surface of Symbiodinium CS-156 cells was previously confirmed. Here we report the crystal structures of SLL-2 and its GalNAc complex as the first crystal structures of a lectin involved in the symbiosis between coral and dinoflagellate. N-Linked sugar chains and a galactose derivative binding site common to H-type lectins were observed in each monomer of the hexameric SLL-2 crystal structure. In addition, unique sugar-binding site-like regions were identified at the top and bottom of the hexameric SLL-2 structure. These structural features suggest a possible binding mode between SLL-2 and Forssman antigen-like pentasaccharide. |
著作権等: | This is a pre-copyedited, author-produced PDF of an article accepted for publication in 'Glycobiology' following peer review. The version of record [Glycobiology (2015) 25 (9): 1016-1023. doi: 10.1093/glycob/cwv033. First published online: May 28, 2015] is available online at: http://glycob.oxfordjournals.org/content/25/9/1016 The full-text file will be made open to the public on May 28, 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/203163 |
DOI(出版社版): | 10.1093/glycob/cwv033 |
PubMed ID: | 26022515 |
出現コレクション: | 学術雑誌掲載論文等 |
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