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dc.contributor.author | Sakurama, Haruko | en |
dc.contributor.author | Kishino, Shigenobu | en |
dc.contributor.author | Mihara, Kousuke | en |
dc.contributor.author | Ando, Akinori | en |
dc.contributor.author | Kita, Keiko | en |
dc.contributor.author | Takahashi, Satomi | en |
dc.contributor.author | Shimizu, Sakayu | en |
dc.contributor.author | Ogawa, Jun | en |
dc.contributor.alternative | 岸野, 重信 | ja |
dc.contributor.alternative | 小川, 順 | ja |
dc.date.accessioned | 2016-02-15T04:14:41Z | - |
dc.date.available | 2016-02-15T04:14:41Z | - |
dc.date.issued | 2014-09 | - |
dc.identifier.issn | 0022-2275 | - |
dc.identifier.uri | http://hdl.handle.net/2433/204519 | - |
dc.description.abstract | The PUFAs include many bioactive lipids. The microbial metabolism of C18 PUFAs is known to produce their bioactive isomers, such as conjugated FAs and hydroxy FAs, but there is little information on that of C20 PUFAs. In this study, we aimed to obtain anaerobic bacteria with the ability to produce novel PUFAs from C20 PUFAs. Through the screening of ∼100 strains of anaerobic bacteria, Clostridium bifermentans JCM 1386 was selected as a strain with the ability to saturate PUFAs during anaerobic cultivation. This strain converted arachidonic acid (cis-5, cis-8, cis-11, cis-14-eicosatetraenoic acid) and EPA (cis-5, cis-8, cis-11, cis-14, cis-17-EPA) into cis-5, cis-8, trans-13-eicosatrienoic acid and cis-5, cis-8, trans-13, cis-17-eicosatetraenoic acid, giving yields of 57% and 67% against the added PUFAs, respectively. This is the first report of the isolation of a bacterium transforming C20 PUFAs into corresponding non-methylene-interrupted FAs. We further investigated the substrate specificity of the biohydrogenation by this strain and revealed that it can convert two cis double bonds at the ω6 and ω9 positions in various C18 and C20 PUFAs into a trans double bond at the ω7 position. This study should serve to open up the development of novel potentially bioactive PUFAs. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.rights | This research was originally published in ['Journal of lipid research' September 2014, 55, 1855-1863.] © the American Society for Biochemistry and Molecular Biology | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | arachidonic acid | en |
dc.subject | fatty acid/metabolism | en |
dc.subject | omega-3 fatty acids | en |
dc.subject | lipids/chemistry | en |
dc.subject | diet and dietary lipids | en |
dc.subject | eicosapentaenoic acid | en |
dc.subject | conjugated fatty acid | en |
dc.subject | non-methylene-interrupted fatty acids | en |
dc.subject.mesh | Anaerobiosis | en |
dc.subject.mesh | Arachidonic Acid/metabolism | en |
dc.subject.mesh | Clostridium bifermentans/metabolism | en |
dc.subject.mesh | Eicosapentaenoic Acid/metabolism | en |
dc.subject.mesh | Hydrogenation | en |
dc.subject.mesh | Linoleic Acids/metabolism | en |
dc.title | Biohydrogenation of C(20) polyunsaturated fatty acids by anaerobic bacteria. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00701215 | - |
dc.identifier.jtitle | Journal of lipid research | en |
dc.identifier.volume | 55 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 1855 | - |
dc.identifier.epage | 1863 | - |
dc.relation.doi | 10.1194/jlr.M045450 | - |
dc.textversion | author | - |
dc.identifier.pmid | 25002034 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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