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dc.contributor.author | Takada, Naoto | en |
dc.contributor.author | Takatsu, Hiroyuki | en |
dc.contributor.author | Miyano, Rie | en |
dc.contributor.author | Nakayama, Kazuhisa | en |
dc.contributor.author | Shin, Hye-Won | en |
dc.contributor.alternative | 申, 惠媛 | ja |
dc.date.accessioned | 2016-02-24T08:01:54Z | - |
dc.date.available | 2016-02-24T08:01:54Z | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 0022-2275 | - |
dc.identifier.uri | http://hdl.handle.net/2433/207262 | - |
dc.description.abstract | Type IV P-type ATPases (P4-ATPases) translocate phospholipids from the exoplasmic to the cytoplasmic leaflets of cellular membranes. We and others previously showed that ATP11C, a member of the P4-ATPases, translocates phosphatidylserine (PS) at the plasma membrane. Twenty years ago, the UPS-1 (uptake of fluorescent PS analogs) cell line was isolated from mutagenized Chinese hamster ovary (CHO)-K1 cells with a defect in nonendocytic uptake of nitrobenzoxadiazole PS. Due to its defect in PS uptake, the UPS-1 cell line has been used in an assay for PS-flipping activity; however, the gene(s) responsible for the defect have not been identified to date. Here, we found that the mRNA level of ATP11C was dramatically reduced in UPS-1 cells relative to parental CHO-K1 cells. By contrast, the level of ATP11A, another PS-flipping P4-ATPase at the plasma membrane, or CDC50A, which is essential for delivery of most P4-ATPases to the plasma membrane, was not affected in UPS-1 cells. Importantly, we identified a nonsense mutation in the ATP11C gene in UPS-1 cells, indicating that the intact ATP11C protein is not expressed. Moreover, exogenous expression of ATP11C can restore PS uptake in UPS-1 cells. These results indicate that lack of the functional ATP11C protein is responsible for the defect in PS uptake in UPS-1 cells and ATP11C is crucial for PS flipping in CHO-K1 cells. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.rights | This research was originally published in [The Journal of Lipid Research, 56, 2151-2157. doi: 10.1194/jlr.M062547] © the American Society for Biochemistry and Molecular Biology | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.title | ATP11C mutation is responsible for the defect in phosphatidylserine uptake in UPS-1 cells. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00701215 | - |
dc.identifier.jtitle | Journal of lipid research | en |
dc.identifier.volume | 56 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 2151 | - |
dc.identifier.epage | 2157 | - |
dc.relation.doi | 10.1194/jlr.M062547 | - |
dc.textversion | author | - |
dc.identifier.pmid | 26420878 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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