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|---|---|---|---|---|
| srep18177.pdf | 1.67 MB | Adobe PDF | 見る/開く |
| タイトル: | Synergistic effect of ATP for RuvA-RuvB-Holliday junction DNA complex formation. |
| 著者: | Iwasa, Takuma Han, Yong-Woon Hiramatsu, Ryo Yokota, Hiroaki Nakao, Kimiko Yokokawa, Ryuji    https://orcid.org/0000-0002-6306-2693 (unconfirmed)Ono, Teruo Harada, Yoshie |
| 著者名の別形: | 原田, 慶恵 |
| 発行日: | 14-Dec-2015 |
| 出版者: | Nature Publishing Group |
| 誌名: | Scientific reports |
| 巻: | 5 |
| 論文番号: | 18177 |
| 抄録: | The Escherichia coli RuvB hexameric ring motor proteins, together with RuvAs, promote branch migration of Holliday junction DNA. Zero mode waveguides (ZMWs) constitute of nanosized holes and enable the visualization of a single fluorescent molecule under micromolar order of the molecules, which is applicable to characterize the formation of RuvA-RuvB-Holliday junction DNA complex. In this study, we used ZMWs and counted the number of RuvBs binding to RuvA-Holliday junction DNA complex. Our data demonstrated that different nucleotide analogs increased the amount of Cy5-RuvBs binding to RuvA-Holliday junction DNA complex in the following order: no nucleotide, ADP, ATPγS, and mixture of ADP and ATPγS. These results suggest that not only ATP binding to RuvB but also ATP hydrolysis by RuvB facilitates a stable RuvA-RuvB-Holliday junction DNA complex formation. |
| 著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| URI: | http://hdl.handle.net/2433/207635 |
| DOI(出版社版): | 10.1038/srep18177 |
| PubMed ID: | 26658024 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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