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Title: Synergistic effect of ATP for RuvA-RuvB-Holliday junction DNA complex formation.
Authors: Iwasa, Takuma
Han, Yong-Woon
Hiramatsu, Ryo
Yokota, Hiroaki
Nakao, Kimiko
Yokokawa, Ryuji  kyouindb  KAKEN_id  orcid (unconfirmed)
Ono, Teruo  kyouindb  KAKEN_id
Harada, Yoshie
Author's alias: 原田, 慶恵
Issue Date: 14-Dec-2015
Publisher: Nature Publishing Group
Journal title: Scientific reports
Volume: 5
Thesis number: 18177
Abstract: The Escherichia coli RuvB hexameric ring motor proteins, together with RuvAs, promote branch migration of Holliday junction DNA. Zero mode waveguides (ZMWs) constitute of nanosized holes and enable the visualization of a single fluorescent molecule under micromolar order of the molecules, which is applicable to characterize the formation of RuvA-RuvB-Holliday junction DNA complex. In this study, we used ZMWs and counted the number of RuvBs binding to RuvA-Holliday junction DNA complex. Our data demonstrated that different nucleotide analogs increased the amount of Cy5-RuvBs binding to RuvA-Holliday junction DNA complex in the following order: no nucleotide, ADP, ATPγS, and mixture of ADP and ATPγS. These results suggest that not only ATP binding to RuvB but also ATP hydrolysis by RuvB facilitates a stable RuvA-RuvB-Holliday junction DNA complex formation.
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
DOI(Published Version): 10.1038/srep18177
PubMed ID: 26658024
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