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dc.contributor.authorTamura, Kentaroja
dc.contributor.authorKawabayashi, Takenorija
dc.contributor.authorShikanai, Toshiharuja
dc.contributor.authorHara-Nishimura, Ikukoja
dc.contributor.alternative西村, いくこja
dc.description.abstractALADIN is a component of the nuclear pore complex in higher eukaryotes. An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a TDNA insertion in one gene can have effects on the expression of neighboring genes.ja
dc.publisherPublic Library of Scienceja
dc.rights© 2016 Tamura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ja
dc.titleDecreased expression of a gene caused by a T-DNA insertion in an adjacent gene in arabidopsisja
dc.type.niitypeJournal Articleja
dc.identifier.jtitlePLOS ONEja
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