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dc.contributor.author | Sakaguchi, Hideya | en |
dc.contributor.author | Kadoshima, Taisuke | en |
dc.contributor.author | Soen, Mika | en |
dc.contributor.author | Narii, Nobuhiro | en |
dc.contributor.author | Ishida, Yoshihito | en |
dc.contributor.author | Ohgushi, Masatoshi | en |
dc.contributor.author | Takahashi, Jun | en |
dc.contributor.author | Eiraku, Mototsugu | en |
dc.contributor.author | Sasai, Yoshiki | en |
dc.contributor.alternative | 坂口, 秀哉 | ja |
dc.contributor.alternative | 高橋, 淳 | ja |
dc.date.accessioned | 2016-04-15T07:28:35Z | - |
dc.date.available | 2016-04-15T07:28:35Z | - |
dc.date.issued | 2015-11-17 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/2433/210235 | - |
dc.description.abstract | The developing dorsomedial telencephalon includes the medial pallium, which goes on to form the hippocampus. Generating a reliable source of human hippocampal tissue is an important step for cell-based research into hippocampus-related diseases. Here we show the generation of functional hippocampal granule- and pyramidal-like neurons from self-organizing dorsomedial telencephalic tissue using human embryonic stem cells (hESCs). First, we develop a hESC culture method that utilizes bone morphogenetic protein (BMP) and Wnt signalling to induce choroid plexus, the most dorsomedial portion of the telencephalon. Then, we find that titrating BMP and Wnt exposure allowed the self-organization of medial pallium tissues. Following long-term dissociation culture, these dorsomedial telencephalic tissues give rise to Zbtb20+/Prox1+ granule neurons and Zbtb20+/KA1+ pyramidal neurons, both of which were electrically functional with network formation. Thus, we have developed an in vitro model that recapitulates human hippocampus development, allowing the generation of functional hippocampal granule- and pyramidal-like neurons. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | en |
dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Biological sciences | en |
dc.subject | Cell biology | en |
dc.subject | Developmental biology | en |
dc.title | Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Nature Communications | en |
dc.identifier.volume | 6 | - |
dc.relation.doi | 10.1038/ncomms9896 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 8896 | - |
dc.identifier.pmid | 26573335 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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