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タイトル: Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes.
著者: Tomura, Michio
Hata, Akihiro
Matsuoka, Satoshi
Shand, Francis H W
Nakanishi, Yasutaka
Ikebuchi, Ryoyo
Ueha, Satoshi
Tsutsui, Hidekazu
Inaba, Kayo
Matsushima, Kouji
Miyawaki, Atsushi
Kabashima, Kenji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-0773-0554 (unconfirmed)
Watanabe, Takeshi
Kanagawa, Osami
著者名の別形: 戸村, 道夫
発行日: 12-Aug-2014
出版者: Nature Publishing Group
誌名: Scientific reports
巻: 4
論文番号: 6030
抄録: Skin-derived dendritic cells (DCs) play a crucial role in the maintenance of immune homeostasis due to their role in antigen trafficking from the skin to the draining lymph nodes (dLNs). To quantify the spatiotemporal regulation of skin-derived DCs in vivo, we generated knock-in mice expressing the photoconvertible fluorescent protein KikGR. By exposing the skin or dLN of these mice to violet light, we were able to label and track the migration and turnover of endogenous skin-derived DCs. Langerhans cells and CD103(+)DCs, including Langerin(+)CD103(+)dermal DCs (DDCs), remained in the dLN for 4-4.5 days after migration from the skin, while CD103(-)DDCs persisted for only two days. Application of a skin irritant (chemical stress) induced a transient >10-fold increase in CD103(-)DDC migration from the skin to the dLN. Tape stripping (mechanical injury) induced a long-lasting four-fold increase in CD103(-)DDC migration to the dLN and accelerated the trafficking of exogenous protein antigens by these cells. Both stresses increased the turnover of CD103(-)DDCs within the dLN, causing these cells to die within one day of arrival. Therefore, CD103(-)DDCs act as sentinels against skin invasion that respond with increased cellular migration and antigen trafficking from the skin to the dLNs.
著作権等: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
URI: http://hdl.handle.net/2433/210293
DOI(出版社版): 10.1038/srep06030
PubMed ID: 25112380
出現コレクション:学術雑誌掲載論文等

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