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タイトル: | Exoproteome analysis of Clostridium cellulovorans in natural soft-biomass degradation |
著者: | Esaka, Kohei Aburaya, Shunsuke Morisaka, Hironobu Kuroda, Kouichi Ueda, Mitsuyoshi |
著者名の別形: | 黒田, 浩一 植田, 充美 |
キーワード: | Clostridium cellulovorans Cellulosome Soft-biomass degradation Proteome analysis Monolithic column |
発行日: | 24-Jan-2015 |
出版者: | SpringerOpen |
誌名: | AMB Express |
巻: | 5 |
論文番号: | 2 |
抄録: | Clostridium cellulovorans is an anaerobic, cellulolytic bacterium, capable of effectively degrading various types of soft biomass. Its excellent capacity for degradation results from optimization of the composition of the protein complex (cellulosome) and production of non-cellulosomal proteins according to the type of substrates. In this study, we performed a quantitative proteome analysis to determine changes in the extracellular proteins produced by C. cellulovorans for degradation of several types of natural soft biomass. C. cellulovorans was cultured in media containing bagasse, corn germ, rice straw (natural soft biomass), or cellobiose (control). Using an isobaric tag method and a liquid chromatograph equipped with a long monolithic silica capillary column/mass spectrometer, we identified 372 proteins in the culture supernatant. Of these, we focused on 77 saccharification-related proteins of both cellulosomal and non-cellulosomal origins. Statistical analysis showed that 18 of the proteins were specifically produced during degradation of types of natural soft biomass. Interestingly, the protein Clocel_3197 was found and commonly involved in the degradation of every natural soft biomass studied. This protein may perform functions, in addition to its known metabolic functions, that contribute to effective degradation of natural soft biomass. |
著作権等: | © 2015, Esaka et al.; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
URI: | http://hdl.handle.net/2433/210479 |
DOI(出版社版): | 10.1186/s13568-014-0089-9 |
PubMed ID: | 25642399 |
出現コレクション: | 学術雑誌掲載論文等 |
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