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Title: Effects of hydrostatic pressure on carcinogenic properties of epithelia
Authors: Tokuda, Shinsaku
Kim, Young Hak  kyouindb  KAKEN_id
Matsumoto, Hisako  kyouindb  KAKEN_id
Muro, Shigeo  kyouindb  KAKEN_id
Hirai, Toyohiro  kyouindb  KAKEN_id
Mishima, Michiaki
Furuse, Mikio
Author's alias: 松本, 久子
室, 繁郎
平井, 豊博
三嶋, 理晃
Issue Date: 30-Dec-2015
Publisher: Public Library of Science
Journal title: PLOS ONE
Volume: 10
Issue: 12
Thesis number: e0145522
Abstract: The relationship between chronic inflammation and cancer is well known. The inflammation increases the permeability of blood vessels and consequently elevates pressure in the interstitial tissues. However, there have been only a few reports on the effects of hydrostatic pressure on cultured cells, and the relationship between elevated hydrostatic pressure and cell properties related to malignant tumors is less well understood. Therefore, we investigated the effects of hydrostatic pressure on the cultured epithelial cells seeded on permeable filters. Surprisingly, hydrostatic pressure from basal to apical side induced epithelial stratification in Madin-Darby canine kidney (MDCK) I and Caco-2 cells, and cavities with microvilli and tight junctions around their surfaces were formed within the multi-layered epithelia. The hydrostatic pressure gradient also promoted cell proliferation, suppressed cell apoptosis, and increased transepithelial ion permeability. The inhibition of protein kinase A (PKA) promoted epithelial stratification by the hydrostatic pressure whereas the activation of PKA led to suppressed epithelial stratification. These results indicate the role of the hydrostatic pressure gradient in the regulation of various epithelial cell functions. The findings in this study may provide clues for the development of a novel strategy for the treatment of the carcinoma.
Rights: © 2015 Tokuda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/210542
DOI(Published Version): 10.1371/journal.pone.0145522
PubMed ID: 26716691
Appears in Collections:Journal Articles

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