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Title: Human and murine APOBEC3s restrict replication of koala retrovirus by different mechanisms
Authors: Nitta, Takayuki
Ha, Dat
Galvez, Felipe
Miyazawa, Takayuki  kyouindb  KAKEN_id
Fan, Hung
Author's alias: 宮沢, 孝幸
Keywords: KoRV
APOBEC3
Glyco-gag
Issue Date: 8-Aug-2015
Publisher: BioMed Central Ltd.
Journal title: Retrovirology
Volume: 12
Thesis number: 68
Abstract: Background: Koala retrovirus (KoRV) is an endogenous and exogenous retrovirus of koalas that may cause lymphoma. As for many other gammaretroviruses, the KoRV genome can potentially encode an alternate form of Gag protein, glyco-gag. Results: In this study, a convenient assay for assessing KoRV infectivity in vitro was employed: the use of DERSE cells (initially developed to search for infectious xenotropic murine leukemia-like viruses). Using infection of DERSE and other human cell lines (HEK293T), no evidence for expression of glyco-gag by KoRV was found, either in expression of glyco-gag protein or changes in infectivity when the putative glyco-gag reading frame was mutated. Since glyco-gag mediates resistance of Moloney murine leukemia virus to the restriction factor APOBEC3, the sensitivity of KoRV (wt or putatively mutant for glyco-gag) to restriction by murine (mA3) or human APOBEC3s was investigated. Both mA3 and hA3G potently inhibited KoRV infectivity. Interestingly, hA3G restriction was accompanied by extensive G → A hypermutation during reverse transcription while mA3 restriction was not. Glyco-gag status did not affect the results. Conclusions: These results indicate that the mechanisms of APOBEC3 restriction of KoRV by hA3G and mA3 differ (deamination dependent vs. independent) and glyco-gag does not play a role in the restriction.
Rights: © 2015 Nitta et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made
URI: http://hdl.handle.net/2433/213937
DOI(Published Version): 10.1186/s12977-015-0193-1
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