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Title: Monoclonal antibody with conformational specificity for a toxic conformer of amyloid β42 and its application toward the Alzheimer's disease diagnosis.
Authors: Murakami, Kazuma  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-3152-1784 (unconfirmed)
Tokuda, Maki
Suzuki, Takashi
Irie, Yumi
Hanaki, Mizuho
Izuo, Naotaka
Monobe, Yoko
Akagi, Ken-Ichi
Ishii, Ryotaro
Tatebe, Harutsugu
Tokuda, Takahiko
Maeda, Masahiro
Kume, Toshiaki
Shimizu, Takahiko
Irie, Kazuhiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7109-8568 (unconfirmed)
Author's alias: 村上, 一馬
徳田, 真樹
鈴木, 天志
入江, 由美
花木, 瑞穂
泉尾, 直孝
物部, 容子
赤木, 謙一
石井, 亮太郎
建部, 陽嗣
徳田, 隆彦
前田, 雅弘
久米, 利明
清水, 孝彦
入江, 一浩
Issue Date: 4-Jul-2016
Publisher: Springer Nature
Journal title: Scientific reports
Volume: 6
Thesis number: 29038
Abstract: Amyloid β-protein (Aβ42) oligomerization is an early event in Alzheimer's disease (AD). Current diagnostic methods using sequence-specific antibodies against less toxic fibrillar and monomeric Aβ42 run the risk of overdiagnosis. Hence, conformation-specific antibodies against neurotoxic Aβ42 oligomers have garnered much attention for developing more accurate diagnostics. Antibody 24B3, highly specific for the toxic Aβ42 conformer that has a turn at Glu22 and Asp23, recognizes a putative Aβ42 dimer, which forms stable and neurotoxic oligomers more potently than the monomer. 24B3 significantly rescues Aβ42-induced neurotoxicity, whereas sequence-specific antibodies such as 4G8 and 82E1, which recognizes the N-terminus, do not. The ratio of toxic to total Aβ42 in the cerebrospinal fluid of AD patients is significantly higher than in control subjects as measured by sandwich ELISA using antibodies 24B3 and 82E1. Thus, 24B3 may be useful for AD diagnosis and therapy.
Description: アルツハイマー病特有のアミロイドβ立体構造に特異的な抗体の開発―より正確な診断手法への応用に期待―. 京都大学プレスリリース. 2016-07-06.
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/215876
DOI(Published Version): 10.1038/srep29038
PubMed ID: 27374357
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2016/160704_1.html
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