|Title:||Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis|
|Authors:||Shiraishi, Hideaki https://orcid.org/0000-0002-3490-9269 (unconfirmed)|
|Author's alias:||白石, 英秋|
|Publisher:||Taylor and Francis Group|
|Journal title:||Bioscience, Biotechnology, and Biochemistry|
|Abstract:||Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately −1 °C min[−1] in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately −1 °C min[−1] in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains.|
|Rights:||This is an Accepted Manuscript of an article published by Taylor & Francis in 'Bioscience, Biotechnology, and Biochemistry' on 31 May 2016, available online: http://www.tandfonline.com/10.1080/09168451.2016.1189320.|
The full-text file will be made open to the public on 31 May 2017 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
|Appears in Collections:||Journal Articles |
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