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Title: Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis
Authors: Shiraishi, Hideaki  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 白石, 英秋
Keywords: cyanobacteria
dietary supplements
Arthrospira platensis
Issue Date: 31-May-2016
Publisher: Taylor and Francis Group
Journal title: Bioscience, Biotechnology, and Biochemistry
Volume: 80
Start page: 2051
End page: 2057
Abstract: Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately −1 °C min[−1] in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately −1 °C min[−1] in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in 'Bioscience, Biotechnology, and Biochemistry' on 31 May 2016, available online:
The full-text file will be made open to the public on 31 May 2017 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1080/09168451.2016.1189320
PubMed ID: 27240586
Appears in Collections:Journal Articles

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