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タイトル: | Extracellular recordings of patterned human pluripotent stem cell-derived cardiomyocytes on aligned fibers |
著者: | Li, Junjun Minami, Itsunari Yu, Leqian Tsuji, Kiyotaka Nakajima, Minako Qiao, Jing Suzuki, Masato Shimono, Ken Nakatsuji, Norio Kotera, Hitetoshi Liu, Li Chen, Yong |
著者名の別形: | 南, 一成 中島, 美奈子 中辻, 憲夫 小寺, 秀俊 劉, 莉 陳, 勇 |
発行日: | 2016 |
出版者: | Hindawi Limited |
誌名: | Stem Cells International |
巻: | 2016 |
論文番号: | 2634013 |
抄録: | Human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) hold high potential for use in drug assessment and myocardial regeneration. To create tissue-like constructs of CMs for extracellular monitoring, we placed aligned fibers (AFs) on the surface of a microelectrode array and then seeded hiPSC-CMs for subsequent monitoring for 14 days. As expected, the CMs organized into anisotropic and matured tissue and the extracellular recordings showed reduced premature beating higher signal amplitude and a higher probability of T-wave detection as compared to the culture without fibers. The CMs on the aligned fibers samples also exhibited anisotropic propagation of the field potential. These results therefore suggest that the hiPSC-CMs cultured on AFs can be used more reliably for cell based assays. |
著作権等: | © 2016 Junjun Li et al. |
URI: | http://hdl.handle.net/2433/216413 |
DOI(出版社版): | 10.1155/2016/2634013 |
PubMed ID: | 27446217 |
出現コレクション: | 学術雑誌掲載論文等 |
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