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Title: Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones.
Authors: Tohya, Mari
Watanabe, Takayasu
Maruyama, Fumito  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-2347-616X (unconfirmed)
Arai, Sakura
Ota, Atsushi
Athey, Taryn B. T.
Fittipaldi, Nahuel
Nakagawa, Ichiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6552-1702 (unconfirmed)
Sekizaki, Tsutomu
Author's alias: 丸山, 史人
Issue Date: 19-Jul-2016
Publisher: Public Library of Science
Journal title: PLOS ONE
Volume: 11
Issue: 7
Thesis number: e0159558
Abstract: Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs), ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels) ranging from one to 10[4] bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated) bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes.
Rights: © 2016 Tohya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/216459
DOI(Published Version): 10.1371/journal.pone.0159558
PubMed ID: 27433935
Appears in Collections:Journal Articles

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