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Title: MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells.
Authors: Parr, Callum J C
Katayama, Shota
Miki, Kenji  kyouindb  KAKEN_id
Kuang, Yi
Yoshida, Yoshinori  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5511-9090 (unconfirmed)
Morizane, Asuka  kyouindb  KAKEN_id
Takahashi, Jun
Yamanaka, Shinya  kyouindb  KAKEN_id
Saito, Hirohide
Author's alias: 片山, 翔太
三木, 健嗣
吉田, 善紀
森実, 飛鳥
髙橋, 淳
山中, 伸弥
齊藤, 博英
Issue Date: 9-Sep-2016
Publisher: Springer Nature
Journal title: Scientific reports
Volume: 6
Thesis number: 32532
Abstract: The efficiency of pluripotent stem cell differentiation is highly variable, often resulting in heterogeneous populations that contain undifferentiated cells. Here we developed a sensitive, target-specific, and general method for removing undesired cells before transplantation. MicroRNA-302a-5p (miR-302a) is highly and specifically expressed in human pluripotent stem cells and gradually decreases to basal levels during differentiation. We synthesized a new RNA tool, miR-switch, as a live-cell reporter mRNA for miR-302a activity that can specifically detect human induced pluripotent stem cells (hiPSCs) down to a spiked level of 0.05% of hiPSCs in a heterogeneous population and can prevent teratoma formation in an in vivo tumorigenicity assay. Automated and selective hiPSC-elimination was achieved by controlling puromycin resistance using the miR-302a switch. Our system uniquely provides sensitive detection of pluripotent stem cells and partially differentiated cells. In addition to its ability to eliminate undifferentiated cells, miR-302a switch also holds great potential in investigating the dynamics of differentiation and/or reprograming of live-cells based on intracellular information.
Description: iPS細胞を選択的に識別・分離・除去できるしくみを合成RNAを用いて構築. 京都大学プレスリリース. 2016-09-09.
Rights: © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/216544
DOI(Published Version): 10.1038/srep32532
PubMed ID: 27608814
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2016/160909_1.html
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