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dc.contributor.author | Yamada, Hisatsugu | en |
dc.contributor.author | Kameda, Tetsuro | en |
dc.contributor.author | Kimura, Yu | en |
dc.contributor.author | Imai, Hirohiko | en |
dc.contributor.author | Matsuda, Tetsuya | en |
dc.contributor.author | Sando, Shinsuke | en |
dc.contributor.author | Toshimitsu, Akio | en |
dc.contributor.author | Aoyama, Yasuhiro | en |
dc.contributor.author | Kondo, Teruyuki | en |
dc.contributor.alternative | 今井, 宏彦 | ja |
dc.contributor.alternative | 松田, 哲也 | ja |
dc.date.accessioned | 2016-09-28T04:25:40Z | - |
dc.date.available | 2016-09-28T04:25:40Z | - |
dc.date.issued | 2016-04-01 | - |
dc.identifier.issn | 2191-1363 | - |
dc.identifier.uri | http://hdl.handle.net/2433/216667 | - |
dc.description.abstract | In an attempt to monitor μm-level trace constituents, we applied here 1H-{13C-15N} triple-resonance nuclear magnetic resonance (NMR) to 13C/15N-enriched l-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μm-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered l-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7-76 μm, which covers the typical stimulated level of -30 μm) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7 T MR machine for small animals. This work suggests that μm-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atom(s) and their appropriate 13C/15N-enrichment is synthetically accessible. Traces via triple resonance! Tripleresonance high-resolution NMR with a cryogenic probe at 16.4 T is sensitive and selective enough to perform quantitative metabolic/pharmacokinetic analysis of l-Dopa/dopamine in brain and liver extracts from mice. This work suggests that μm-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atoms and their appropriate 13C/15N-enrichment is synthetically accessible. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-VCH Verlag | en |
dc.rights | © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en |
dc.subject | dopamine | en |
dc.subject | L-dopa | en |
dc.subject | metabolic analysis | en |
dc.subject | neurotransmitters | en |
dc.subject | stable isotope enrichment | en |
dc.subject | triple-resonance NMR | en |
dc.title | 13C/15N-Enriched l -Dopa as a Triple-Resonance NMR Probe to Monitor Neurotransmitter Dopamine in the Brain and Liver Extracts of Mice | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | ChemistryOpen | en |
dc.identifier.volume | 5 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 125 | - |
dc.identifier.epage | 128 | - |
dc.relation.doi | 10.1002/open.201500196 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 27308224 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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