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Title: Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes
Authors: Ogiso, Satoshi
Yasuchika, Kentaro
Fukumitsu, Ken
Ishii, Takamichi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7461-9653 (unconfirmed)
Kojima, Hidenobu
Miyauchi, Yuya
Yamaoka, Ryoya
Komori, Junji
Katayama, Hokahiro
Kawai, Takayuki
Yoshitoshi, Elena Yukie
Kita, Sadahiko
Yasuda, Katsutaro
Uemoto, Shinji  kyouindb  KAKEN_id
Author's alias: 安近, 健太郎
上本, 伸二
Issue Date: 21-Oct-2016
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 6
Thesis number: 35887
Abstract: A whole-organ regeneration approach, using a decellularised xenogeneic liver as a scaffold for the construction of a transplantable liver was recently reported. Deriving suitable scaffolds was the first step towards clinical application; however, effective recellularisation remains to be achieved. This report presents a strategy for the improvement of the recellularisation process, using novel cell-seeding technique and cell source. We evaluated recellularised liver grafts repopulated through the portal vein or the biliary duct with mice adult hepatocytes or E14.5 foetal hepatocytes. More than 80% of the cells seeded through the biliary tree entered the parenchyma beyond the ductule-lining matrix barrier and distributed throughout the liver lobule. In contrast, about 20% of the cells seeded through the portal tree entered the parenchyma. The gene expression levels of foetal hepatocyte albumin, glucose 6-phosphatase, transferrin, cytokeratin 19, and gamma-glutamyl transpeptidase were increased in three-dimensional cultures in the native liver-derived scaffolds, and the activation of liver detoxification enzymes and formation of biliary duct-like structures were supported. The metabolic functions of liver grafts recellularised with different cell types were similar. These results suggest that biliary tree cell-seeding approach is promising, and that liver progenitor cells represent a good cell source candidate.
Rights: © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/217251
DOI(Published Version): 10.1038/srep35887
PubMed ID: 27767181
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