ダウンロード数: 218
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.celrep.2016.11.026.pdf | 11.5 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Ishikura, Yukiko | en |
dc.contributor.author | Yabuta, Yukihiro | en |
dc.contributor.author | Ohta, Hiroshi | en |
dc.contributor.author | Hayashi, Katsuhiko | en |
dc.contributor.author | Nakamura, Tomonori | en |
dc.contributor.author | Okamoto, Ikuhiro | en |
dc.contributor.author | Yamamoto, Takuya | en |
dc.contributor.author | Kurimoto, Kazuki | en |
dc.contributor.author | Shirane, Kenjiro | en |
dc.contributor.author | Sasaki, Hiroyuki | en |
dc.contributor.author | Saitou, Mitinori | en |
dc.contributor.alternative | 石藏, 友紀子 | ja |
dc.contributor.alternative | 斎藤, 通紀 | ja |
dc.date.accessioned | 2016-12-07T06:09:17Z | - |
dc.date.available | 2016-12-07T06:09:17Z | - |
dc.date.issued | 2016-12-06 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | http://hdl.handle.net/2433/217467 | - |
dc.description | マウス多能性幹細胞から精子幹細胞を試験管内で誘導 : 精子形成全過程の試験管内誘導の基盤形成. 京都大学プレスリリース. 2016-12-07. | ja |
dc.description.abstract | The in vitro derivation and propagation of spermatogonial stem cells (SSCs) from pluripotent stem cells (PSCs) is a key goal in reproductive science. We show here that when aggregated with embryonic testicular somatic cells (reconstituted testes), primordial germ cell-like cells (PGCLCs) induced from mouse embryonic stem cells differentiate into spermatogonia-like cells in vitro and are expandable as cells that resemble germline stem cells (GSCs), a primary cell line with SSC activity. Remarkably, GSC-like cells (GSCLCs), but not PGCLCs, colonize adult testes and, albeit less effectively than GSCs, contribute to spermatogenesis and fertile offspring. Whole-genome analyses reveal that GSCLCs exhibit aberrant methylation at vulnerable regulatory elements, including those critical for spermatogenesis, which may restrain their spermatogenic potential. Our study establishes a strategy for the in vitro derivation of SSC activity from PSCs, which, we propose, relies on faithful epigenomic regulation. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | en |
dc.rights | © 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en |
dc.subject | spermatogonial stem cells | en |
dc.subject | embryonic stem cells | en |
dc.subject | primordial germ cells | en |
dc.subject | testes | en |
dc.subject | DNA methylation | en |
dc.subject | epigenetic reprogramming | en |
dc.title | In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Cell Reports | en |
dc.identifier.volume | 17 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 2789 | - |
dc.identifier.epage | 2804 | - |
dc.relation.doi | 10.1016/j.celrep.2016.11.026 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 27926879 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2016-12-07-2 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
![](/dspace/image/articlelinker.gif)
このリポジトリに保管されているアイテムはすべて著作権により保護されています。