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dc.contributor.authorYamamoto, Satoshien
dc.contributor.authorMasuda, Reijien
dc.contributor.authorSato, Yukutoen
dc.contributor.authorSado, Tetsuyaen
dc.contributor.authorAraki, Hitoshien
dc.contributor.authorKondoh, Michioen
dc.contributor.authorMinamoto, Toshifumien
dc.contributor.authorMiya, Masakien
dc.contributor.alternative山本, 哲史ja
dc.contributor.alternative益田, 玲爾ja
dc.contributor.alternative荒木, 仁志ja
dc.contributor.alternative近藤, 倫生ja
dc.contributor.alternative源, 利文ja
dc.contributor.alternative宮, 正樹ja
dc.descriptionわずか1日の調査で魚種の8割を検出 : 海水からのDNA解析法で. 京都大学プレスリリース. 2017-01-13.ja
dc.description.abstractEnvironmental DNA (eDNA) metabarcoding has emerged as a potentially powerful tool to assess aquatic community structures. However, the method has hitherto lacked field tests that evaluate its effectiveness and practical properties as a biodiversity monitoring tool. Here, we evaluated the ability of eDNA metabarcoding to reveal fish community structures in species-rich coastal waters. High-performance fish-universal primers and systematic spatial water sampling at 47 stations covering ~11 km2 revealed the fish community structure at a species resolution. The eDNA metabarcoding based on a 6-h collection of water samples detected 128 fish species, of which 62.5% (40 species) were also observed by underwater visual censuses conducted over a 14-year period. This method also detected other local fishes (≥23 species) that were not observed by the visual censuses. These eDNA metabarcoding features will enhance marine ecosystem-related research, and the method will potentially become a standard tool for surveying fish communities.en
dc.publisherSpringer Natureen
dc.rights© The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
dc.titleEnvironmental DNA metabarcoding reveals local fish communities in a species-rich coastal seaen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleScientific Reportsen
dcterms.accessRightsopen access-
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