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dc.contributor.authorGoto, Kazuyaen
dc.contributor.authorImamura, Keikoen
dc.contributor.authorKomatsu, Kenichien
dc.contributor.authorMitani, Kohnosukeen
dc.contributor.authorAiba, Kazuhiroen
dc.contributor.authorNakatsuji, Norioen
dc.contributor.authorInoue, Makotoen
dc.contributor.authorKawata, Akihiroen
dc.contributor.authorYamashita, Hirofumien
dc.contributor.authorTakahashi, Ryosukeen
dc.contributor.authorInoue, Haruhisaen
dc.contributor.alternative後藤, 和也ja
dc.contributor.alternative今村, 恵子ja
dc.contributor.alternative小松, 研一ja
dc.contributor.alternative井上, 治久ja
dc.date.accessioned2017-02-07T06:37:03Z-
dc.date.available2017-02-07T06:37:03Z-
dc.date.issued2017-03-17-
dc.identifier.issn2329-0501-
dc.identifier.urihttp://hdl.handle.net/2433/217986-
dc.descriptionセンダイウイルスベクターを用いてES細胞/iPS細胞から脊髄運動ニューロンを簡便に作製する技術開発. 京都大学プレスリリース. 2017-02-07.ja
dc.description.abstractAmyotrophic lateral sclerosis (ALS) is a progressive and fatal degenerative disorder of motor neurons (MNs). Embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs) now help us to understand the pathomechanisms of ALS via disease modeling. Various methods to differentiate ESCs/iPSCs into MNs by the addition of signaling molecules have been reported. However, classical methods require multiple steps, and newer simple methods using the transduction of transcription factors run the risk of genomic integration of the vector genes. Heterogeneity of the expression levels of the transcription factors also remains an issue. Here we describe a novel approach for differentiating human and mouse ESCs/iPSCs into MNs using a single Sendai virus vector encoding three transcription factors, LIM/homeobox protein 3, neurogenin 2, and islet-1, which are integration free. This single-vector method, generating HB9-positive cells on day 2 from human iPSCs, increases the ratio of MNs to neurons compared to the use of three separate Sendai virus vectors. In addition, the MNs derived via this method from iPSCs of ALS patients and model mice display disease phenotypes. This simple approach significantly reduces the efforts required to generate MNs, and it provides a useful tool for disease modeling.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier B.V.en
dc.rights© 2017 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectmotor neuronsen
dc.subjectSendai virusen
dc.subjectinduced pluripotent stem cellsen
dc.subjectembryonic stem cellsen
dc.subjectiPSCen
dc.subjectESCen
dc.subjectdifferentiationen
dc.subjectdirect conversionen
dc.subjecttranscription factoren
dc.titleSimple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectorsen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleMolecular Therapy - Methods & Clinical Developmenten
dc.identifier.volume4-
dc.identifier.spage115-
dc.identifier.epage125-
dc.relation.doi10.1016/j.omtm.2016.12.007-
dc.textversionpublisher-
dc.identifier.pmid28344997-
dc.relation.urlhttps://www.kyoto-u.ac.jp/ja/research-news/2017-02-07-
dcterms.accessRightsopen access-
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