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dc.contributor.authorGoto, Kazuyaja
dc.contributor.authorImamura, Keikoja
dc.contributor.authorKomatsu, Kenichija
dc.contributor.authorMitani, Kohnosukeja
dc.contributor.authorAiba, Kazuhiroja
dc.contributor.authorNakatsuji, Norioja
dc.contributor.authorInoue, Makotoja
dc.contributor.authorKawata, Akihiroja
dc.contributor.authorYamashita, Hirofumija
dc.contributor.authorTakahashi, Ryosukeja
dc.contributor.authorInoue, Haruhisaja
dc.contributor.alternative後藤, 和也ja
dc.contributor.alternative今村, 恵子ja
dc.contributor.alternative小松, 研一ja
dc.contributor.alternative井上, 治久ja
dc.date.accessioned2017-02-07T06:37:03Z-
dc.date.available2017-02-07T06:37:03Z-
dc.date.issued2017-03-17ja
dc.identifier.issn2329-0501ja
dc.identifier.urihttp://hdl.handle.net/2433/217986-
dc.descriptionセンダイウイルスベクターを用いてES細胞/iPS細胞から脊髄運動ニューロンを簡便に作製する技術開発. 京都大学プレスリリース. 2017-02-07.ja
dc.description.abstractAmyotrophic lateral sclerosis (ALS) is a progressive and fatal degenerative disorder of motor neurons (MNs). Embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs) now help us to understand the pathomechanisms of ALS via disease modeling. Various methods to differentiate ESCs/iPSCs into MNs by the addition of signaling molecules have been reported. However, classical methods require multiple steps, and newer simple methods using the transduction of transcription factors run the risk of genomic integration of the vector genes. Heterogeneity of the expression levels of the transcription factors also remains an issue. Here we describe a novel approach for differentiating human and mouse ESCs/iPSCs into MNs using a single Sendai virus vector encoding three transcription factors, LIM/homeobox protein 3, neurogenin 2, and islet-1, which are integration free. This single-vector method, generating HB9-positive cells on day 2 from human iPSCs, increases the ratio of MNs to neurons compared to the use of three separate Sendai virus vectors. In addition, the MNs derived via this method from iPSCs of ALS patients and model mice display disease phenotypes. This simple approach significantly reduces the efforts required to generate MNs, and it provides a useful tool for disease modeling.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevier B.V.ja
dc.rights© 2017 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).ja
dc.subjectmotor neuronsja
dc.subjectSendai virusja
dc.subjectinduced pluripotent stem cellsja
dc.subjectembryonic stem cellsja
dc.subjectiPSCja
dc.subjectESCja
dc.subjectdifferentiationja
dc.subjectdirect conversionja
dc.subjecttranscription factorja
dc.titleSimple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectorsja
dc.type.niitypeJournal Articleja
dc.identifier.jtitleMolecular Therapy - Methods & Clinical Developmentja
dc.identifier.volume4ja
dc.identifier.spage115ja
dc.identifier.epage125ja
dc.relation.doi10.1016/j.omtm.2016.12.007ja
dc.textversionpublisherja
dc.relation.urlhttp://www.kyoto-u.ac.jp/ja/research/research_results/2016/170202_2.htmlja
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