このアイテムのアクセス数: 52

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
srep43134.pdf3.17 MBAdobe PDF見る/開く
タイトル: Mechanical diagnosis of human erythrocytes by ultra-high speed manipulation unraveled critical time window for global cytoskeletal remodeling
著者: Ito, Hiroaki
Murakami, Ryo
Sakuma, Shinya
Tsai, Chia-Hung Dylan
Gutsmann, Thomas
Brandenburg, Klaus
Pöschl, Johannes M. B.
Arai, Fumihito
Kaneko, Makoto
Tanaka, Motomu
著者名の別形: 新井, 史人
金子, 真
田中, 求
発行日: 24-Feb-2017
出版者: Springer Nature
誌名: Scientific Reports
巻: 7
論文番号: 43134
抄録: Large deformability of erythrocytes in microvasculature is a prerequisite to realize smooth circulation. We develop a novel tool for the three-step "Catch-Load-Launch" manipulation of a human erythrocyte based on an ultra-high speed position control by a microfluidic "robotic pump". Quantification of the erythrocyte shape recovery as a function of loading time uncovered the critical time window for the transition between fast and slow recoveries. The comparison with erythrocytes under depletion of adenosine triphosphate revealed that the cytoskeletal remodeling over a whole cell occurs in 3 orders of magnitude longer timescale than the local dissociation-reassociation of a single spectrin node. Finally, we modeled septic conditions by incubating erythrocytes with endotoxin, and found that the exposure to endotoxin results in a significant delay in the characteristic transition time for cytoskeletal remodeling. The high speed manipulation of erythrocytes with a robotic pump technique allows for high throughput mechanical diagnosis of blood-related diseases.
記述: ヒト赤血球変形の時間スケールを解明 : ロボットポンプが新たな細胞機能の解明・診断の糸口に. 京都大学プレスリリース. 2017-02-27.
著作権等: © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/218417
DOI(出版社版): 10.1038/srep43134
PubMed ID: 28233788
関連リンク: http://www.kyoto-u.ac.jp/ja/research/research_results/2016/170224_1.html
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。