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タイトル: Identification of neurexophilin 3 as a novel supportive factor for survival of induced pluripotent stem cell-derived dopaminergic progenitors
著者: Nishimura, Kaneyasu
Murayama, Shigeo
Takahashi, Jun  kyouindb  KAKEN_id
著者名の別形: 西村, 周泰
村山, 繁雄
高橋, 淳
キーワード: Neurexophilin 3
Brain environment
Cell transplantation therapy
Parkinson's disease
Induced pluripotent stem cells
1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine
発行日: Aug-2015
出版者: Wiley-Blackwell
誌名: Stem Cells Translational Medicine
巻: 4
号: 8
開始ページ: 932
終了ページ: 944
抄録: Successful cell transplantation for Parkinson’s disease (PD) depends on both an optimal host brain environment and ideal donor cells. We report that a secreted peptide, neurexophilin 3 (NXPH3), supports the survival of mouse induced pluripotent stem cell-derived (iPSC-derived) dopaminergic (DA) neurons in vitro and in vivo. We compared the gene expression profiles in the mouse striatum from two different environments: a supportive environment, which we defined as 1 week after acute administration of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP), and a nonsupportive environment, defined as 8 weeks after chronic administration of MPTP. NXPH3 expression was higher in the former condition and lower in the latter compared with untreated controls. When we injected mouse iPSC-derived neural cells along withNXPH3 into themouse striatum, the ratio of tyrosine hydroxylasepositive DA neurons per graft volume was higher at 8 weeks compared with cell injections that excluded NXPH3. In addition, quantitative polymerase chain reaction analyses of the postmortem putamen revealed that the expression level of NXPH3 was lower in PD patients compared with normal controls. These findings will contribute to optimizing the host brain environment and patient recruitment in cell therapy for PD.
著作権等: © AlphaMed Press. This article is made available under the terms of the Creative Commons Attribution License.
URI: http://hdl.handle.net/2433/218481
DOI(出版社版): 10.5966/sctm.2014-0197
PubMed ID: 26041738
出現コレクション:学術雑誌掲載論文等

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