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タイトル: | Behavior of leucine-rich repeat-containing G-protein coupled receptor 5-expressing cells in the reprogramming process |
著者: | Arioka, Yuko Ito, Hiroyasu Hirata, Akihiro Semi, Katsunori Yamada, Yasuhiro Seishima, Mitsuru |
著者名の別形: | 山田, 泰広 |
キーワード: | Leucine-rich repeat-containing G-protein coupled receptor 5 Cell reprogramming Induced pluripotent stem cells |
発行日: | Apr-2017 |
出版者: | Elsevier BV |
誌名: | Stem Cell Research |
巻: | 20 |
開始ページ: | 1 |
終了ページ: | 9 |
抄録: | It remains unclear what cells are proper for the generation of induced pluripotent stem cells (iPSCs). Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) is well known as a tissue stem cell and progenitor marker, both of which are reported to be sensitive to reprogramming. In the present study, we examined the reprogramming behavior of Lgr5-expressing cells (Lgr5 + cells). First, we compared reprogramming behavior using mouse Lgr5 + and Lgr5 negative (Lgr5 −) hair follicles (HFs). The number of alkaline phosphatase staining-positive cells was lesser in a well of Lgr5 + HFs than in Lgr5 − HFs; however, the ratio of Nanog + SSEA1 + cells in the cell mixture derived from Lgr5 + HFs was much higher than that from Lgr5 − HFs. Lgr5 + cells could be induced from mouse embryonic fibroblasts (MEFs) after transduction with Yamanaka factors. As shown in HFs, the progeny of Lgr5 + cells arising from MEFs highly converted into Nanog + cells and did not form Nanog − colonies. The progeny represented the status of the late reprogramming phase to a higher degree than the nonprogeny. We also confirmed this using human Lg5 + cells. Our findings suggest that the use of Lgr5 + cells will minimize sorting efforts for obtaining superior iPSCs. |
著作権等: | © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/218685 |
DOI(出版社版): | 10.1016/j.scr.2017.01.012 |
PubMed ID: | 28192743 |
出現コレクション: | 学術雑誌掲載論文等 |
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