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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-017-00264-y.pdf | 2.32 MB | Adobe PDF | 見る/開く |
| タイトル: | New platform for simple and rapid protein-based affinity reactions |
| 著者: | Kubota, Kei Kubo, Takuya   https://orcid.org/0000-0002-9274-3295 (unconfirmed)Tanigawa, Tetsuya Naito, Toyohiro Otsuka, Koji https://orcid.org/0000-0003-1088-0569 (unconfirmed) |
| 著者名の別形: | 久保田, 圭 久保, 拓也 谷川, 哲也 内藤, 豊裕 大塚, 浩二 |
| 発行日: | 14-Mar-2017 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 7 |
| 号: | 1 |
| 論文番号: | 178 |
| 抄録: | We developed a spongy-like porous polymer (spongy monolith) consisting of poly (ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate. |
| 記述: | タンパク質間反応のための新規プラットフォーム : 抗体分離の簡便化・高速化・低コスト化に向けて. 京都大学プレスリリース. 2017-03-15. |
| 著作権等: | © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| URI: | http://hdl.handle.net/2433/218919 |
| DOI(出版社版): | 10.1038/s41598-017-00264-y |
| PubMed ID: | 28282970 |
| 関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-03-15 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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