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Title: New platform for simple and rapid protein-based affinity reactions
Authors: Kubota, Kei
Kubo, Takuya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-9274-3295 (unconfirmed)
Tanigawa, Tetsuya
Naito, Toyohiro
Otsuka, Koji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-1088-0569 (unconfirmed)
Author's alias: 久保田, 圭
久保, 拓也
谷川, 哲也
内藤, 豊裕
大塚, 浩二
Issue Date: 14-Mar-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Issue: 1
Thesis number: 178
Abstract: We developed a spongy-like porous polymer (spongy monolith) consisting of poly (ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.
Description: タンパク質間反応のための新規プラットフォーム : 抗体分離の簡便化・高速化・低コスト化に向けて. 京都大学プレスリリース. 2017-03-15.
Rights: © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/218919
DOI(Published Version): 10.1038/s41598-017-00264-y
PubMed ID: 28282970
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2017-03-15
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