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タイトル: Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal
著者: Funabashi, Teruki
Katoh, Yohei  kyouindb  KAKEN_id
Michisaka, Saki
Terada, Masaya
Sugawa, Maho
Nakayama, Kazuhisa  kyouindb  KAKEN_id
著者名の別形: 加藤, 洋平
中山, 和久
発行日: 1-Mar-2017
出版者: American Society for Cell Biology
誌名: Molecular Biology of the Cell
巻: 28
号: 5
開始ページ: 624
終了ページ: 633
抄録: Cilia function as cellular antennae to sense and transduce extracellular signals. A number of proteins are specifically localized in cilia. Anterograde and retrograde ciliary protein trafficking are mediated by the IFT-B and IFT-A complexes in concert with kinesin-2 and dynein-2 motors, respectively. However, the role of KIF17, a homodimeric kinesin-2 protein, in protein trafficking has not been fully understood in vertebrate cilia. In this study, we demonstrated, by using the visible immunoprecipitation assay, that KIF17 interacts with the IFT46-IFT56 dimer in the IFT-B complex through its C-terminal sequence located immediately upstream of the nuclear localization signal (NLS). We then showed that KIF17 reaches the ciliary tip independently of its motor domain and requires IFT-B binding for its entry into cilia rather than for its intraciliary trafficking. We further showed that KIF17 ciliary entry depends not only on its binding to IFT-B but also on its NLS, to which importin α proteins bind. Taking the results together, we conclude that in mammalian cells, KIF17 is dispensable for ciliogenesis and IFT-B trafficking but requires IFT-B, as well as its NLS, for its ciliary entry across the permeability barrier located at the ciliary base.
著作権等: © 2017 Funabashi, Katoh, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
許諾条件により本文は2017-06-01に公開
URI: http://hdl.handle.net/2433/218975
DOI(出版社版): 10.1091/mbc.E16-09-0648
出現コレクション:学術雑誌掲載論文等

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