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Title: The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
Authors: Tanaka, Yoshiki
Ono, Natsuki
Shima, Takahiro
Tanaka, Gaku
Katoh, Yohei  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-1649-4917 (unconfirmed)
Nakayama, Kazuhisa  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7701-7183 (unconfirmed)
Takatsu, Hiroyuki
Shin, Hye Won
Author's alias: 加藤, 洋平
中山, 和久
申, 惠媛
Issue Date: 1-Dec-2016
Publisher: American Society for Cell Biology
Journal title: Molecular Biology of the Cell
Volume: 27
Issue: 24
Start page: 3883
End page: 3893
Abstract: Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane.
Rights: © 2016 Tanaka, Ono, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc -sa/3.0).
URI: http://hdl.handle.net/2433/218976
DOI(Published Version): 10.1091/mbc.E16-08-0586
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