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タイトル: | Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs |
著者: | Kinoshita, Masanao Suzuki, Kenichi G.N. Matsumori, Nobuaki Takada, Misa Ano, Hikaru Morigaki, Kenichi Abe, Mitsuhiro Makino, Asami Kobayashi, Toshihide Hirosawa, Koichiro M. Fujiwara, Takahiro K. Kusumi, Akihiro Murata, Michio |
著者名の別形: | 木下, 祥尚 鈴木, 健一 松森, 信明 楠見, 明弘 村田, 道雄 |
発行日: | 22-Mar-2017 |
出版者: | Rockefeller University Press |
誌名: | The Journal of Cell Biology |
巻: | 216 |
号: | 4 |
開始ページ: | 1183 |
終了ページ: | 1204 |
抄録: | Sphingomyelin (SM) has been proposed to form cholesterol-dependent raft domains and sphingolipid domains in the plasma membrane (PM). How SM contributes to the formation and function of these domains remains unknown, primarily because of the scarcity of suitable fluorescent SM analogs. We developed new fluorescent SM analogs by conjugating a hydrophilic fluorophore to the SM choline headgroup without eliminating its positive charge, via a hydrophilic nonaethylene glycol linker. The new analogs behaved similarly to the native SM in terms of their partitioning behaviors in artificial liquid order-disorder phase-separated membranes and detergent-resistant PM preparations. Single fluorescent molecule tracking in the live-cell PM revealed that they indirectly interact with each other in cholesterol- and sphingosine backbone{textendash}dependent manners, and that, for ~{}10{textendash}50 ms, they undergo transient colocalization-codiffusion with a glycosylphosphatidylinositol (GPI)-anchored protein, CD59 (in monomers, transient-dimer rafts, and clusters), in CD59-oligomer size{textendash}, cholesterol-, and GPI anchoring{textendash}dependent manners. These results suggest that SM continually and rapidly exchanges between CD59-associated raft domains and the bulk PM. |
記述: | 脂質の挙動をありのままに再現する蛍光プローブでラフトの形成機構を解明. 京都大学プレスリリース. 2017-03-28. |
著作権等: | © 2017 Kinoshita et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http ://www .rupress .org /terms /). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https ://creativecommons .org /licenses /by -nc -sa /4 .0 /). |
URI: | http://hdl.handle.net/2433/219127 |
DOI(出版社版): | 10.1083/jcb.201607086 |
PubMed ID: | 28330937 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-03-28-0 |
出現コレクション: | 学術雑誌掲載論文等 |
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