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bio.023473.pdf | 2.33 MB | Adobe PDF | 見る/開く |
タイトル: | Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules |
著者: | Miyamoto, Kei Tajima, Yosuke Yoshida, Koki Oikawa, Mami Azuma, Rika Allen, George E. Tsujikawa, Tomomi Tsukaguchi, Tomomasa Bradshaw, Charles R. Jullien, Jerome Yamagata, Kazuo Matsumoto, Kazuya Anzai, Masayuki Imai, Hiroshi Gurdon, John B. Yamada, Masayasu ![]() |
著者名の別形: | 宮本, 圭 山田, 雅保 |
キーワード: | Nuclear transfer Reprogramming Epigenetic modification Mouse |
発行日: | 2017 |
出版者: | The Company of Biologists |
誌名: | Biology Open |
巻: | 6 |
開始ページ: | 415 |
終了ページ: | 424 |
抄録: | Animal cloning has been achieved in many species by transplanting differentiated cell nuclei to unfertilized oocytes. However, the low efficiencies of cloning have remained an unresolved issue. Here we find that the combination of two small molecules, trichostatin A (TSA) and vitamin C (VC), under culture condition with bovine serum albumin deionized by ion-exchange resins, dramatically improves the cloning efficiency in mice and 15% of cloned embryos develop to term by means of somatic cell nuclear transfer (SCNT). The improvement was not observed by adding the non-treated, rather than deionized, bovine serum. RNA-seq analyses of SCNT embryos at the two-cell stage revealed that the treatment with TSA and VC resulted in the upregulated expression of previously identified reprogramming-resistant genes. Moreover, the expression of early-embryo-specific retroelements was upregulated by the TSA and VC treatment. The enhanced gene expression was relevant to the VC-mediated reduction of histone H3 lysine 9 methylation in SCNT embryos. Our study thus shows a simply applicable method to greatly improve mouse cloning efficiency, and furthers our understanding of how somatic nuclei acquire totipotency. |
記述: | クローン動物の発生率の劇的な改善に成功--培養条件の改善のみで安定したクローンマウスの作出が可能に--. 京都大学プレスリリース. 2017-04-17. |
著作権等: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
URI: | http://hdl.handle.net/2433/219613 |
DOI(出版社版): | 10.1242/bio.023473 |
PubMed ID: | 28412714 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-04-17 |
出現コレクション: | 学術雑誌掲載論文等 |
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