ダウンロード数: 198
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s12862-016-0783-0.pdf | 780.53 kB | Adobe PDF | 見る/開く |
タイトル: | Variation in ligand responses of the bitter taste receptors TAS2R1 and TAS2R4 among New World monkeys |
著者: | Tsutsui, Kei Otoh, Masahiro Sakurai, Kodama Suzuki-Hashido, Nami Hayakawa, Takashi Misaka, Takumi Ishimaru, Yoshiro Aureli, Filippo Melin, Amanda D. Kawamura, Shoji Imai, Hiroo |
著者名の別形: | 筒井, 圭 鈴木-橋戸, 南美 早川, 卓志 今井, 啓雄 |
キーワード: | Bitter taste receptor TAS2R G protein-coupled receptor New World monkey Interspecific functional variation Molecular evolution |
発行日: | 12-Oct-2016 |
出版者: | Springer Nature |
誌名: | BMC Evolutionary Biology |
巻: | 16 |
論文番号: | 208 |
抄録: | Background New World monkeys (NWMs) are unique in that they exhibit remarkable interspecific variation in color vision and feeding behavior, making them an excellent model for studying sensory ecology. However, it is largely unknown whether non-visual senses co-vary with feeding ecology, especially gustation, which is expected to be indispensable in food selection. Bitter taste, which is mediated by bitter taste receptors (TAS2Rs) in the tongue, helps organisms avoid ingesting potentially toxic substances in food. In this study, we compared the ligand sensitivities of the TAS2Rs of five species of NWMs by heterologous expression in HEK293T cells and calcium imaging. Results We found that TAS2R1 and TAS2R4 orthologs differ in sensitivity among the NWM species for colchicine and camphor, respectively. We then reconstructed the ancestral receptors of NWM TAS2R1 and TAS2R4, measured the evolutionary shift in ligand sensitivity, and identified the amino acid replacement at residue 62 as responsible for the high sensitivity of marmoset TAS2R4 to colchicine. Conclusions Our results provide a basis for understanding the differences in feeding ecology among NWMs with respect to bitter taste. |
著作権等: | © 2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
URI: | http://hdl.handle.net/2433/219655 |
DOI(出版社版): | 10.1186/s12862-016-0783-0 |
PubMed ID: | 27733116 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。