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タイトル: | Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch. |
著者: | Hirosawa, Moe Fujita, Yoshihiko https://orcid.org/0000-0001-6321-2121 (unconfirmed) Parr, Callum J C Hayashi, Karin Kashida, Shunnichi Hotta, Akitsu https://orcid.org/0000-0002-2619-7441 (unconfirmed) Woltjen, Knut https://orcid.org/0000-0003-2293-1183 (unconfirmed) Saito, Hirohide |
著者名の別形: | 弘澤, 萌 齊藤, 博英 |
発行日: | 27-Jul-2017 |
出版者: | Oxford University Press |
誌名: | Nucleic acids research |
巻: | 45 |
号: | 13 |
論文番号: | e118 |
抄録: | The CRISPR-Cas9 system is a powerful genome-editing tool useful in a variety of biotechnology and biomedical applications. Here we developed a synthetic RNA-based, microRNA (miRNA)-responsive CRISPR-Cas9 system (miR-Cas9 switch) in which the genome editing activity of Cas9 can be modulated through endogenous miRNA signatures in mammalian cells. We created miR-Cas9 switches by using a miRNA-complementary sequence in the 5΄-UTR of mRNA encoding Streptococcus pyogenes Cas9. The miR-21-Cas9 or miR-302-Cas9 switches selectively and efficiently responded to miR-21-5p in HeLa cells or miR-302a-5p in human induced pluripotent stem cells, and post-transcriptionally attenuated the Cas9 activity only in the target cells. Moreover, the miR-Cas9 switches could differentially control the genome editing by sensing endogenous miRNA activities within a heterogeneous cell population. Our miR-Cas9 switch system provides a promising framework for cell-type selective genome editing and cell engineering based on intracellular miRNA information. |
記述: | 細胞種に応じてゲノム編集を制御する技術を開発. 京都大学プレスリリース. 2017-05-22. |
著作権等: | © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
URI: | http://hdl.handle.net/2433/224940 |
DOI(出版社版): | 10.1093/nar/gkx309 |
PubMed ID: | 28525578 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-05-22-0 |
出現コレクション: | 学術雑誌掲載論文等 |
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