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タイトル: | Involvement of AMPK in regulating slow-twitch muscle atrophy during hindlimb unloading in mice |
著者: | Egawa, Tatsuro https://orcid.org/0000-0001-9363-1589 (unconfirmed) Goto, Ayumi Ohno, Yoshitaka Yokoyama, Shingo Ikuta, Akihiro Suzuki, Miho Sugiura, Takao Ohira, Yoshinobu Yoshioka, Toshitada Hayashi, Tatsuya https://orcid.org/0000-0001-7600-4735 (unconfirmed) Goto, Katsumasa |
著者名の別形: | 江川, 達郎 林, 達也 |
キーワード: | AMP-activated protein kinase protein degradation autophagy ubiquitin-proteasome microRNA heat shock protein |
発行日: | 1-Oct-2015 |
出版者: | American Physiological Society |
誌名: | American Journal of Physiology - Endocrinology And Metabolism |
巻: | 309 |
号: | 7 |
開始ページ: | E651 |
終了ページ: | E662 |
抄録: | AMPK is considered to have a role in regulating skeletal muscle mass. However, there are no studies investigating the function of AMPK in modulating skeletal muscle mass during atrophic conditions. In the present study, we investigated the difference in unloading-associated muscle atrophy and molecular functions in response to 2-wk hindlimb suspension between transgenic mice overexpressing the dominant-negative mutant of AMPK (AMPK-DN) and their wild-type (WT) littermates. Male WT (n = 24) and AMPK-DN (n = 24) mice were randomly divided into two groups: an untreated preexperimental control group (n = 12 in each group) and an unloading (n = 12 in each group) group. The relative soleus muscle weight and fiber cross-sectional area to body weight were decreased by ∼30% in WT mice by hindlimb unloading and by ∼20% in AMPK-DN mice. There were no changes in puromycin-labeled protein or Akt/70-kDa ribosomal S6 kinase signaling, the indicators of protein synthesis. The expressions of ubiquitinated proteins and muscle RING finger 1 mRNA and protein, markers of the ubiquitin-proteasome system, were increased by hindlimb unloading in WT mice but not in AMPK-DN mice. The expressions of molecules related to the protein degradation system, phosphorylated forkhead box class O3a, inhibitor of κBα, microRNA (miR)-1, and miR-23a, were decreased only in WT mice in response to hindlimb unloading, and 72-kDa heat shock protein expression was higher in AMPK-DN mice than in WT mice. These results imply that AMPK partially regulates unloading-induced atrophy of slow-twitch muscle possibly through modulation of the protein degradation system, especially the ubiquitin-proteasome system. |
著作権等: | © 2015 the American Physiological Society. This is the accepted version of the article, which has been published in final form at http://doi.org/10.1152/ajpendo.00165.2015 This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/225059 |
DOI(出版社版): | 10.1152/ajpendo.00165.2015 |
PubMed ID: | 26244519 |
出現コレクション: | 学術雑誌掲載論文等 |
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