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タイトル: FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5′-DNA end
著者: Sato, Koichi
Shimomuki, Mayo
Katsuki, Yoko
Takahashi, Daisuke
Kobayashi, Wataru
Ishiai, Masamichi
Miyoshi, Hiroyuki
Takata, Minoru
Kurumizaka, Hitoshi
著者名の別形: 髙田, 穣
発行日: Dec-2016
出版者: Oxford University Press (OUP)
誌名: Nucleic Acids Research
巻: 44
号: 22
開始ページ: 10758
終了ページ: 10771
抄録: The FANCI-FANCD2 (I-D) complex is considered to work with RAD51 to protect the damaged DNA in the stalled replication fork. However, the means by which this DNA protection is accomplished have remained elusive. In the present study, we found that the I-D complex directly binds to RAD51, and stabilizes the RAD51-DNA filament. Unexpectedly, the DNA binding activity of FANCI, but not FANCD2, is explicitly required for the I-D complex-mediated RAD51-DNA filament stabilization. The RAD51 filament stabilized by the I-D complex actually protects the DNA end from nucleolytic degradation by an FA-associated nuclease, FAN1. This DNA end protection is not observed with the RAD51 mutant from FANCR patient cells. These results clearly answer the currently enigmatic question of how RAD51 functions with the I-D complex to prevent genomic instability at the stalled replication fork.
著作権等: © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/226279
DOI(出版社版): 10.1093/nar/gkw876
出現コレクション:学術雑誌掲載論文等

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