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| タイトル: | New insight into the dynamical system of αB-crystallin oligomers |
| 著者: | Inoue, Rintaro   Takata, Takumi Fujii, Norihiko Ishii, Kentaro Uchiyama, Susumu Sato, Nobuhiro  https://orcid.org/0000-0002-6960-6567 (unconfirmed)Oba, Yojiro Wood, Kathleen Kato, Koichi Fujii, Noriko Sugiyama, Masaaki |
| 著者名の別形: | 井上, 倫太郎 髙田, 匠 佐藤, 信浩 大場, 洋次郎 藤井, 紀子 杉山, 正明 |
| キーワード: | Chaperones Kinetics |
| 発行日: | 6-Jul-2016 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 6 |
| 論文番号: | 29208 |
| 抄録: | α-Crystallin possesses a dynamic quaternary structure mediated by its subunit dynamics. Elucidation of a mechanism of subunit dynamics in homo-oligomers of αB-crystallin was tackled through deuteration-assisted small-angle neutron scattering (DA-SANS) and electrospray ionization (ESI) native mass spectrometry (nMS). The existence of subunit exchange was confirmed with DA-SANS, and monomers liberated from the oligomers were observed with nMS. With increasing temperature, an increase in both the exchange rate and monomer population was observed despite the absence of oligomer collapse. It is proposed that transiently liberated subunits, namely, “traveling subunits, ” play a role in subunit exchange. Moreover, we propose that protein function is regulated by these traveling subunits. |
| 著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. |
| URI: | http://hdl.handle.net/2433/226356 |
| DOI(出版社版): | 10.1038/srep29208 |
| PubMed ID: | 27381175 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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