ダウンロード数: 140
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.stemcr.2015.07.007.pdf | 5.74 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Chantzoura, Eleni | en |
dc.contributor.author | Skylaki, Stavroula | en |
dc.contributor.author | Menendez, Sergio | en |
dc.contributor.author | Kim, Shin-Il | en |
dc.contributor.author | Johnsson, Anna | en |
dc.contributor.author | Linnarsson, Sten | en |
dc.contributor.author | Woltjen, Knut | en |
dc.contributor.author | Chambers, Ian | en |
dc.contributor.author | Kaji, Keisuke | en |
dc.date.accessioned | 2017-07-14T07:43:39Z | - |
dc.date.available | 2017-07-14T07:43:39Z | - |
dc.date.issued | 2015-09-08 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2433/226438 | - |
dc.description.abstract | Since the first generation of induced pluripotent stem cells (iPSCs), several reprogramming systems have been used to study its molecular mechanisms. However, the system of choice largely affects the reprogramming efficiency, influencing our view on the mechanisms. Here, we demonstrate that reprogramming triggered by less efficient polycistronic reprogramming cassettes not only highlights mesenchymal-to-epithelial transition (MET) as a roadblock but also faces more severe difficulties to attain a pluripotent state even post-MET. In contrast, more efficient cassettes can reprogram both wild-type and Nanog−/− fibroblasts with comparable efficiencies, routes, and kinetics, unlike the less efficient reprogramming systems. Moreover, we attribute a previously reported variation in the N terminus of KLF4 as a dominant factor underlying these critical differences. Our data establish that some reprogramming roadblocks are system dependent, highlighting the need to pursue mechanistic studies with close attention to the systems to better understand reprogramming. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2015 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | en |
dc.title | Reprogramming Roadblocks Are System Dependent | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Stem Cell Reports | en |
dc.identifier.volume | 5 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 350 | - |
dc.identifier.epage | 364 | - |
dc.relation.doi | 10.1016/j.stemcr.2015.07.007 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 26278041 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。