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タイトル: Prevalence and Antimicrobial Susceptibility of Vibrio parahaemolyticus Isolated from Short Mackerels (Rastrelliger brachysoma) in Malaysia
著者: Tan, Chia W.
Malcolm, Tan T. H.
Kuan, Chee H.
Thung, Tze Y.
Chang, Wei S.
Loo, Yuet Y.
Premarathne, Jayasekara M. K. J. K.
Ramzi, Othman B.
Norshafawatie, Mohd F. S.
Yusralimuna, Nordin
Rukayadi, Yaya
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
著者名の別形: 中口, 義次
キーワード: Vibrio parahaemolyticus
finfish
MPN
antibiotic susceptibility
MAR
発行日: 13-Jun-2017
出版者: Frontiers Media SA
誌名: Frontiers in Microbiology
巻: 8
論文番号: 1087
抄録: Numerous prevalence studies and outbreaks of Vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. Information on the quantitative detection of V. parahaemolyticus in finfish species is limited. In this study, short mackerels (Rastrelliger brachysoma) obtained from different retail marketplaces were monitored with the presence of total and pathogenic strains of V. parahaemolyticus. Out of 130 short mackerel samples, 116 (89.2%) were detected with the presence of total V. parahaemolyticus and microbial loads of total V. parahaemolyticus ranging from <3 to >10[5] MPN/g. Prevalence of total V. parahaemolyticus was found highest in wet markets (95.2%) followed by minimarkets (89.1%) and hypermarkets (83.3%). Pathogenic V. parahaemolyticus strains (tdh+ and/or trh+) were detected in 16.2% (21 of 130) of short mackerel samples. The density of tdh+ V. parahaemolyticus strains were examined ranging from 3.6 to >105 MPN/g and microbial loads of V. parahaemolyticus strains positive for both tdh and trh were found ranging from 300 to 740 MPN/g. On the other hand, antibiotic susceptibility profiles of V. parahaemolyticusstrains isolated from short mackerels were determined through disc diffusion method in this study. Assessment of antimicrobial susceptibility profile of V. parahaemolyticusrevealed majority of the isolates were highly susceptible to ampicillin sulbactam, meropenem, ceftazidime, and imipenem, but resistant to penicillin G and ampicillin. Two isolates (2.99%) exhibited the highest multiple antibiotic resistance (MAR) index value of 0.41 which shown resistance to 7 antibiotics. Results of the present study demonstrated that the occurrence of pathogenic V. parahaemolyticus strains in short mackerels and multidrug resistance of V. parahaemolyticus isolates could be a potential public health concerns to the consumer. Furthermore, prevalence data attained from the current study can be further used to develop a microbial risk assessment model to estimate health risks associated with the consumption of short mackerels contaminated with pathogenic V. parahaemolyticus.
著作権等: © 2017 Tan, Malcolm, Kuan, Thung, Chang, Loo, Premarathne, Ramzi, Norshafawatie, Yusralimuna, Rukayadi, Nakaguchi, Nishibuchi and Radu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
URI: http://hdl.handle.net/2433/226643
DOI(出版社版): 10.3389/fmicb.2017.01087
PubMed ID: 28659901
出現コレクション:学術雑誌掲載論文等

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