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Title: Drosophila melanogaster rhodopsin Rh7 is a UV-to-visible light sensor with an extraordinarily broad absorption spectrum
Authors: Sakai, Kazumi
Tsutsui, Kei
Yamashita, Takahiro  kyouindb  KAKEN_id
Iwabe, Naoyuki
Takahashi, Keisuke
Wada, Akimori
Shichida, Yoshinori
Author's alias: 酒井, 佳寿美
筒井, 圭
山下, 高廣
七田, 芳則
Keywords: G protein-coupled receptors
Visual system
Issue Date: 4-Aug-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 7349
Abstract: The genome of Drosophila melanogaster contains seven rhodopsin genes. Rh1-6 proteins are known to have respective absorption spectra and function as visual pigments in ocelli and compound eyes. In contrast, Rh7 protein was recently revealed to function as a circadian photoreceptor in the brain. However, its molecular properties have not been characterized yet. Here we successfully prepared a recombinant protein of Drosophila Rh7 in mammalian cultured cells. Drosophila Rh7 bound both 11-cis-retinal and 11-cis-3-hydroxyretinal to form photo-pigments which can absorb UV light. Irradiation with UV light caused formation of a visible-light absorbing metarhodopsin that activated Gq-type of G protein. This state could be photoconverted back to the original state and, thus Rh7 is a Gq-coupled bistable pigment. Interestingly, Rh7 (lambda max = 350 nm) exhibited an unusual broad spectrum with a longer wavelength tail reaching 500 nm, whose shape is like a composite of spectra of two pigments. In contrast, replacement of lysine at position 90 with glutamic acid caused the formation of a normal-shaped absorption spectrum with maximum at 450 nm. Therefore, Rh7 is a unique photo-sensor that can cover a wide wavelength region by a single pigment to contribute to non-visual photoreception.
Description: ショウジョウバエは脳で紫外光を感じる --一人二役の脳内光受容タンパク質--. 京都大学プレスリリース. 2017-08-18.
Rights: © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/226807
DOI(Published Version): 10.1038/s41598-017-07461-9
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/170804_1.html
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